Oligonucleotide Array-in-Well Platform for Detection and Genotyping Human Adenoviruses by Utilizing Upconverting Phosphor Label Technology

被引：31
作者：
Yliharsila, Minna [1 ,2 ]
Valta, Timo [1 ]
Karp, Maija [1 ]
Hattara, Liisa [3 ]
Harju, Emilia [4 ]
Holsa, Jorma [4 ,5 ,6 ]
Saviranta, Petri [3 ]
Waris, Matti [2 ]
Soukka, Tero [1 ]
机构：
[1] Univ Turku, Dept Biotechnol, FI-20520 Turku, Finland
[2] Univ Turku, Dept Virol, FI-20520 Turku, Finland
[3] VTT Tech Res Ctr Finland, Ctr Med Biotechnol, FI-20520 Turku, Finland
[4] Univ Turku, Dept Chem, FI-20014 Turku, Finland
[5] Univ Turku, Ctr Mat & Surfaces MatSurf, FI-20520 Turku, Finland
[6] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508900 Sao Paulo, Brazil
来源：
ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 04期
关键词：
RESONANCE ENERGY-TRANSFER; HOMOGENEOUS IMMUNOASSAY; FLUORESCENT LABELS; GENE-EXPRESSION; RAPID DETECTION; MICROARRAYS; DNA; NANOPARTICLES; OPTIMIZATION; VIRUS;
D O I：
10.1021/ac103155f
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have developed a robust array-in-well test platform based on an oligonudeotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.
引用
收藏
页码：1456 / 1461
页数：6
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