Isolation and Identification of the DNA Aptamer Target to Acetamiprid

被引：149

作者：

He, Jiang ^{[1
,2
]}

Liu, Yuan ^{[1
]}

Fan, Mingtao ^{[2
]}

Liu, Xianjin ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Food Qual Safety & Detect,Minist Agr, Key Lab Food Qual & Safety Jiangsu Prov, Key Lab Food Safety Monitoring & Management, Nanjing 210014, Jiangsu, Peoples R China

[2] NW A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Aptamer; pesticide; acetamiprid; SELEX; IN-VITRO SELECTION; RNA LIGANDS; AFFINITY; BIND; ENRICHMENT; SELEX; ACID;

D O I：

10.1021/jf104189g

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

As an alternative to antibodies, aptamers have a great potential as analytical tools for pesticide detection. In this work, aptamers targeting acetamiprid were selected by a specific systematic evolution of ligands by exponential enrichment (SELEX) strategy, where a single-stranded DNA (ssDNA) library was immobilized and target modification was eliminated. After 18 rounds of repeated selection, the ssDNA pool was enriched and then 14 sequences were selected and carefully identified. At last, an acetamiprid-specific aptamer with the apparent dissociation constant (K-d) estimated to be 4.98 mu M was successfully obtained. Further work is ongoing to develop an aptamer-based detection method for field determination of this pesticides in agricultural products and environmental samples.

引用

页码：1582 / 1586

页数：5

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