In vitro transcription of a whole gene on a surface-coupled template

被引：8
作者：
Steffen, J
von Nickisch-Rosenegk, M
Bier, FF
机构：
[1] Fraunhofer Inst Biomed Engn, Dept Mol Bioanalyt & Bioelect, D-14558 Nuthetal, Germany
[2] Univ Potsdam, Inst Biochem & Biol, D-14469 Potsdam, Germany
来源：
LAB ON A CHIP | 2005年 / 5卷 / 06期
关键词：
D O I：
10.1039/b501091h
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated.
引用
收藏
页码：665 / 668
页数：4
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