Cellulase activities in biomass conversion: measurement methods and comparison

被引：179

作者：

Dashtban, Mehdi ^{[1
,2
]}

Maki, Miranda ^{[1
,2
]}

Leung, Kam Tin ^{[2
]}

Mao, Canquan ^{[3
]}

Qin, Wensheng ^{[1
,2
]}

机构：

[1] Lakehead Univ, Biorefining Res Initiat, Thunder Bay, ON P7B 5E1, Canada

[2] Lakehead Univ, Dept Biol, Thunder Bay, ON P7B 5E1, Canada

[3] SW Jiaotong Univ, Sch Life Sci & Engn, Chengdu, Peoples R China

来源：

CRITICAL REVIEWS IN BIOTECHNOLOGY | 2010年 / 30卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Biofuel; biomass; bioconversion; cellulase; cellulase assays; FILTER-PAPER ASSAY; EXTRACELLULAR BETA-GLUCOSIDASE; TRICHODERMA-REESEI; PURIFICATION; BIOCONVERSION; CELLOBIOSE; SUBSTRATE; RESIDUES; MIXTURE; SYSTEM;

D O I：

10.3109/07388551.2010.490938

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by beta-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and beta-glucosidases acting in a synergistic manner. In biopolymer hydrolysis studies, enzyme assay is an indispensable part. The most commonly used assays for the individual enzymes as well as total cellulase activity measurements, including their advantages and limitations, are summarized in this review article. In addition, some novel approaches recently used for enzyme assays are summarized.

引用

页码：302 / 309

页数：8

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