Selection of Peptides Binding to HCV E2 and Inhibiting Viral Infectivity

被引：10

作者：

Hong, Hye-Won ^{[1
]}

Lee, Seong-Wook ^{[2
]}

Myung, Heejoon ^{[1
]}

机构：

[1] Hankuk Univ Foreign Studies, Dept Biosci & Biotechnol, Yongin 449791, South Korea

[2] Dankook Univ, Inst Nanosensor & Biotechnol, Dept Mol Biol, Yongin 448701, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2010年 / 20卷 / 12期

关键词：

Hepatitis C virus; E2; CD81; infection; peptide; HEPATITIS-C VIRUS; ENTRY; CD81; RECEPTOR; STEP;

D O I：

10.4014/jmb.1007.07036

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The envelope glycoprotein E2 of hepatitis C virus (HCV) binds to various cell surface receptors for viral infection. We performed biopanning against this protein and selected peptides from phage display peptide libraries. Two short peptides, pep7-1 and pep12-1, were selected and their ability to inhibit the infection process was investigated. When pep7-1 was present, the infectivity of HCV particles in cell culture was notably decreased. This decrease was demonstrated by Western blot analysis, immunofluorescence assay, and reverse transcription PCR assay. However, pep12-1 showed little inhibitory effect on HCV infection.

引用

页码：1769 / 1771

页数：3

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