Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters

Gold nanocluster was synthesized by bovine serum albumin directing, and was characterized by fluorescence spectrophotometer, zeta sizer and non-denaturing polyacrylamide gel electrophoresis. The results show that the gold nanocluster's fluorescence signal is strong and stable at different pH value. The interaction between gold nanoclusters and HeLa cells was further studied. It demonstrates that the gold naoclusters could be successfully uptaken by living cells. The good effects of the fluorescent labeling of living cells can be achieved by optimal incubation time and gold nanoclusters' concentration. Moreover, the fixation treatment did not change the form of labeling. These results provide some guidance to the further application of fluorescent gold nanoclusters in the field of live cells labeling.