Interaction of lysozyme with a tear film lipid layer model: A molecular dynamics simulation study

被引：25

作者：

Wizert, Alicia ^{[1
]}

Iskander, D. Robert ^{[1
]}

Cwiklik, Lukasz ^{[2
]}

机构：

[1] Wroclaw Univ Sci & Technol, Dept Biomed Engn, Fac Fundamental Problems Technol, Wroclaw, Poland

[2] Czech Acad Sci, J Heyrovsky Inst Phys Chem, Vvi, Prague, Czech Republic

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2017年 / 1859卷 / 12期

关键词：

Tear film; Tear film lipid layer; Lysozyme; Molecular dynamics; Lipid-protein interaction; DRY EYE; INTERNATIONAL WORKSHOP; FLUID; LIPOCALIN; SURFACE; SUBCOMMITTEE; DISCOMFORT; STABILITY; MECHANISM; DISEASE;

D O I：

10.1016/j.bbamem.2017.08.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tear film is a thin multilayered structure covering the cornea. Its outermost layer is a lipid film underneath of which resides on an aqueous layer. This tear film lipid layer (TFLL) is itself a complex structure, formed by both polar and nonpolar lipids. It was recently suggested that due to tear film dynamics, TFLL contains in homogeneities in the form of polar lipid aggregates. The aqueous phase of tear film contains lachrymal-origin proteins, whereby lysozyme is the most abundant. These proteins can alter TFLL properties, mainly by reducing its surface tension. However, a detailed nature of protein-lipid interactions in tear film is not known. We investigate the interactions of lysozyme with TFLL in molecular details by employing coarse-grained molecular dynamics simulations. We demonstrate that lysozyme, due to lateral restructuring of TFLL, is able to penetrate the tear lipid film embedded in inverse micellar aggregates.

引用

页码：2289 / 2296

页数：8

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