Dimerization of long hibernation promoting factor from Staphylococcus aureus: Structural analysis and biochemical characterization

被引：6

作者：

Usachev, Konstantin S. ^{[1
]}

Fatkhullin, Bulat F. ^{[1
,2
]}

Klochkova, Evelina A. ^{[1
]}

Miftakhov, Aynur K. ^{[1
]}

Golubev, Alexander A. ^{[1
]}

Bikmullin, Aidar G. ^{[1
]}

Nurullina, Liliya I. ^{[1
]}

Garaeva, Natalia S. ^{[1
]}

Islamov, Daut R. ^{[1
]}

Gabdulkhakov, Azat G. ^{[2
]}

Lekontseva, Natalia V. ^{[2
]}

Tishchenko, Svetlana V. ^{[2
]}

Balobanov, Vitaly A. ^{[2
]}

Khusainov, Iskander Sh. ^{[1
,3
,4
]}

Yusupov, Marat M. ^{[1
,3
]}

Validov, Shamil Z. ^{[1
]}

机构：

[1] Kazan Fed Univ, Inst Fundamental Med cold Biol, Struct Biol Lab, 18 Kremlyovskaya, Kazan 420008, Russia

[2] Russian Acad Sci, Inst Prot Res, Inst Skaya 4, Pushchino 142290, Moscow Region, Russia

[3] Univ Strasbourg, Dept Biol & Genom Struct, Inst Genet & Biol Mol & Cellulaire, CNRS,UMR7104,INSERM,U964, 1 Rue Laurent Fries, F-67400 Illkirch Graffenstaden, France

[4] EMBL Heidelberg, Meyerhofstr 1, D-69117 Heidelberg, Germany

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2020年 / 209卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

Ribosome; Staphylococcus aureus; Long HPF; Hibernation; X-ray; 100S RIBOSOME; PSEUDOMONAS-AERUGINOSA; STATIONARY-PHASE; FACTOR HOMOLOG; YFIA; SURVIVAL; YHBH;

D O I：

10.1016/j.jsb.2019.107408

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Staphylococcus aureus hibernation promoting factor (SaHPF) is responsible for the formation of 100S ribosome dimers, which in turn help this pathogen to reduce energy spent under unfavorable conditions. Ribosome dimer formation strongly depends on the dimerization of the C-terminal domain of SaHPF (CTDSaHPF). In this study, we solved the crystal structure of CTDSaHPF at 1.6 resolution and obtained a precise arrangement of the dimer interface. Residues Phe(160), Val(162), Thr(171), Ile(173), Tyr(175), Ile(185) andThr(187) in the dimer interface of SaHPF protein were mutated and the effects were analyzed for the formation of 100S disomes of ribosomes isolated from S. aureus. It was shown that substitution of any of single residues Phe(160), Val(162), Ile(173), Tyr(175) and Ile(185) in the SaHPF homodimer interface abolished the ribosome dimerization in vitro.

引用

页数：7

共 34 条

[1] PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution [J].

Adams, Paul D. ;

Afonine, Pavel V. ;

Bunkoczi, Gabor ;

Chen, Vincent B. ;

Davis, Ian W. ;

Echols, Nathaniel ;

Headd, Jeffrey J. ;

Hung, Li-Wei ;

Kapral, Gary J. ;

Grosse-Kunstleve, Ralf W. ;

McCoy, Airlie J. ;

Moriarty, Nigel W. ;

Oeffner, Robert ;

Read, Randy J. ;

Richardson, David C. ;

Richardson, Jane S. ;

Terwilliger, Thomas C. ;

Zwart, Peter H. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 :213-221

[2] Ribosome dimerization is essential for the efficient regrowth of Bacillus subtilis [J].

Akanuma, Genki ;

Kazo, Yuka ;

Tagami, Kazumi ;

Hiraoka, Hirona ;

Yano, Koichi ;

Suzuki, Shota ;

Hanai, Ryo ;

Nanamiya, Hideaki ;

Kato-Yamada, Yasuyuki ;

Kawamura, Fujio .

MICROBIOLOGY-SGM, 2016, 162 :448-458

[3] Resuscitation of Pseudomonas aeruginosa from dormancy requires hibernation promoting factor (PA4463) for ribosome preservation [J].

Akiyama, Tatsuya ;

Williamson, Kerry S. ;

Schaefer, Robert ;

Pratt, Shawna ;

Chang, Connie B. ;

Franklin, Michael J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (12) :3204-3209

[4] Alternative sigma factor RpoN and its modulation protein YhbH are indispensable for Erwinia amylovora virulence [J].

Ancona, Veronica ;

Li, Wenting ;

Zhao, Youfu .

MOLECULAR PLANT PATHOLOGY, 2014, 15 (01) :58-66

[5] Ribosome hibernation factor promotes Staphylococcal survival and differentially represses translation [J].

Basu, Arnab ;

Yap, Mee-Ngan F. .

NUCLEIC ACIDS RESEARCH, 2016, 44 (10) :4881-4893

[6] Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1 [J].

Beckert, Bertrand ;

Turk, Martin ;

Czech, Andreas ;

Berninghausen, Otto ;

Beckmann, Roland ;

Ignatova, Zoya ;

Plitzko, Juergen M. ;

Wilson, Daniel N. .

NATURE MICROBIOLOGY, 2018, 3 (10) :1115-+

[7] Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization [J].

Beckert, Bertrand ;

Abdelshahid, Maha ;

Schaefer, Heinrich ;

Steinchen, Wieland ;

Arenz, Stefan ;

Berninghausen, Otto ;

Beckmann, Roland ;

Bange, Gert ;

Turgay, Kursad ;

Wilson, Daniel N. .

EMBO JOURNAL, 2017, 36 (14) :2061-2072

[8] Structure of the chloroplast ribosome with chl-RRF and hibernation-promoting factor [J].

Boerema, Annemarie Perez ;

Aibara, Shintaro ;

Paul, Bijoya ;

Tobiasson, Victor ;

Kimanius, Dari ;

Forsberg, Bjorn O. ;

Wallden, Karin ;

Lindahl, Erik ;

Amunts, A. .

NATURE PLANTS, 2018, 4 (04) :212-217

[9] Coot:: model-building tools for molecular graphics [J].

Emsley, P ;

Cowtan, K .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 :2126-2132

[10] Long-Term Survival of Borrelia burgdorferi Lacking the Hibernation Promotion Factor Homolog in the Unfed Tick Vector [J].

Fazzino, Lisa ;

Tilly, Kit ;

Dulebohn, Daniel P. ;

Rosa, Patricia A. .

INFECTION AND IMMUNITY, 2015, 83 (12) :4800-4810

← 1 2 3 4 →