Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

被引：38

作者：

Hsu, SH

Shyu, HW

Hsieh-Li, HM

Li, H ^{[1
]}

机构：

[1] Acad Sinica, Inst Mol Biol, Taipei 11529, Taiwan

[2] Natl Def Med Ctr, Inst Life Sci, Taipei, Taiwan

[3] Taipei Med Coll, Inst Med Sci, Taipei, Taiwan

来源：

MECHANISMS OF DEVELOPMENT | 2001年 / 100卷 / 02期

关键词：

basic helix-loop-helix; leucine zipper; mouse; spermatogenesis; testis;

D O I：

10.1016/S0925-4773(00)00513-X

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules. (C) 2001 Elsevier Science Ireland Ltd. Ail rights reserved.

引用

页码：177 / 187

页数：11

共 50 条

[41] Characterization of htAKR, a novel gene product in the aldo-keto reductase family specifically expressed in human testis
Azuma, Y
Nishinaka, T
Ushijima, S
Soh, J
Katsuyama, M
Lu, HP
Kawata, M
Yabe-Nishimura, C
Miki, T
MOLECULAR HUMAN REPRODUCTION, 2004, 10 (07) : 527 - 533
[42] hGC-1, a novel human olfactomedin-related protein, specifically expressed in myeloid lineage.
Zhang, JC
Liu, WL
Tang, DC
Chen, L
Wang, M
Pack, SD
Zhuang, ZP
Rogers, GP
BLOOD, 2000, 96 (11) : 608A - +
[43] Characterization of a human regulatory subunit of protein phosphatase 3 gene (PPP3RL) expressed specifically in testis
Liu, LL
Zhang, JX
Yuan, J
Dang, YJ
Yang, CY
Chen, XJ
Xu, JN
Yu, L
MOLECULAR BIOLOGY REPORTS, 2005, 32 (01) : 41 - 45
[44] Characterization of a human regulatory subunit of protein phosphatase 3 gene (PPP3RL) expressed specifically in testis
Lingling Liu
Jianxuan Zhang
Jian Yuan
Yongjun Dang
Chenyi Yang
Xiujuan Chen
Jianing Xu
Long Yu
Molecular Biology Reports, 2005, 32 : 41 - 45
[45] UCHL-1 protein expression specifically marks spermatogonia in wild bovid testis
Sandeep Goel
Ranjeet Singh Mahla
Sanjay Kumar Suman
Niranjan Reddy
Hiroshi Imai
European Journal of Wildlife Research, 2011, 57 : 663 - 667
[46] UCHL-1 protein expression specifically marks spermatogonia in wild bovid testis
Goel, Sandeep
Mahla, Ranjeet Singh
Suman, Sanjay Kumar
Reddy, Niranjan
Imai, Hiroshi
EUROPEAN JOURNAL OF WILDLIFE RESEARCH, 2011, 57 (03) : 663 - 667
[47] MIST1, a novel bHLH transcription factor, is expressed in hematopoietic cells and is regulated by E-box binding protein.
Lemercier, C
Brown, A
Gaffe, J
Konieczny, SF
Reiffers, J
Ripoche, J
BLOOD, 1997, 90 (10) : 1356 - 1356
[48] A NOVEL PUTATIVE G-PROTEIN-COUPLED RECEPTOR HIGHLY EXPRESSED IN LUNG AND TESTIS
EVA, C
SPRENGEL, R
DNA AND CELL BIOLOGY, 1993, 12 (05) : 393 - 399
[49] CYRL, a novel cytokine receptor-like protein expressed in testis, lung, and spleen
Chritton, SL
Sheng, M
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 267 (03) : 697 - 702
[50] A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies
Yasuoka, H
Ihn, H
Medsger, TA
Hirakata, M
Kawakami, Y
Ikeda, Y
Kuwana, M
JOURNAL OF IMMUNOLOGY, 2003, 171 (12): : 6883 - 6890

← 1 2 3 4 5 →