Dynamic complex formation during the yeast cell cycle

被引：314

作者：

de Lichtenberg, U

Jensen, LJ

Brunak, S

Bork, P ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

[2] Tech Univ Denmark, Ctr Biol Sequence Anal, DK-2800 Lyngby, Denmark

[3] Max Delbruck Ctr Mol Med, D-13092 Berlin, Germany

来源：

SCIENCE | 2005年 / 307卷 / 5710期

关键词：

D O I：

10.1126/science.1105103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To analyze the dynamics of protein complexes during the yeast cell cycle, we integrated data on protein interactions and gene expression. The resulting time-dependent interaction network places both periodically and constitutively expressed proteins in a temporal cell cycle context, thereby revealing previously unknown components and modules. We discovered that most complexes consist of both periodically and constitutively expressed subunits, which suggests that the former control complex activity by a mechanism of just-in-time assembly. Consistent with this, we show that additional regulation through targeted degradation and phosphorylation by Cdc28p (Cdk1) specifically affects the periodically expressed proteins.

引用

页码：724 / 727

页数：4

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