Isolation from Ochrobactrum anthropi of a Novel Class II 5-Enopyruvylshikimate-3-Phosphate Synthase with High Tolerance to Glyphosate

被引：32

作者：

Tian, Yong-Sheng ^{[1
]}

Xiong, Ai-Sheng ^{[1
]}

Xu, Jing ^{[1
]}

Zhao, Wei ^{[1
]}

Gao, Feng ^{[1
]}

Fu, Xiao-Yan ^{[1
]}

Xu, Hu ^{[1
]}

Zheng, Jian-Li ^{[1
]}

Peng, Ri-He ^{[1
]}

Yao, Quan-Hong ^{[1
]}

机构：

[1] Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 17期

关键词：

5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; 3-PHOSPHATE SYNTHASE; ESCHERICHIA-COLI; HERBICIDE GLYPHOSATE; CATALYTIC RESIDUES; DIRECTED EVOLUTION; SHIKIMATE PATHWAY; GENE; IDENTIFICATION; AROA;

D O I：

10.1128/AEM.00770-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.

引用

页码：6001 / 6005

页数：5

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