Successful pregnancy and delivery after calcium ionophore oocyte activation in a normozoospermic patient with previous repeated failed fertilization after intracytoplasmic sperm injection

被引：78

作者：

Eldar-Geva, T ^{[1
]}

Brooks, B ^{[1
]}

Margalioth, EJ ^{[1
]}

Zylber-Haran, E ^{[1
]}

Gal, M ^{[1
]}

Silber, SJ ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, IVF Unit, Shaare Zedek Med Ctr, IL-91031 Jerusalem, Israel

来源：

FERTILITY AND STERILITY | 2003年 / 79卷

关键词：

calcium ionophore; failed fertilization; ICSI; oocyte activation;

D O I：

10.1016/S0015-0282(03)00369-8

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To describe a successful pregnancy and delivery after calcium ionophore oocyte activation in a normozoospermic patient with previous repeated failed fertilization after intracytoplasmic sperm injection (ICSI). Design: Case report. Setting: In vitro fertilization unit in a university affiliated medical center. Patient(s): A couple with 5 years of unexplained primary infertility who had repeated failed fertilization after ICSI. Intervention(s): Controlled ovarian hyperstimulation, oocyte pick-up, ICSI, assisted oocyte activation with calcium ionophore, embryo culture, and ET. Main Outcome Measure(s): Fertilization rate, implantation, pregnancy, and delivery. Result(s): Assisted oocyte activation with calcium ionophore A23187 after ICSI resulted in reasonable fertilization rates in three cycles (4/6, 5/16 and 7/20 oocytes). Two pregnancies were achieved; the first ended with second trimester miscarriage due to fetal anomaly and the second with a delivery of three healthy babies. Conclusion(s): Calcium ionophore oocyte activation seems to be a useful method in cases of repeated failed fertilization after ICSI.

引用

页码：1656 / 1658

页数：3

共 19 条

[1] Intracytoplasmic sperm injection and conventional in vitro fertilization for sibling oocytes in cases of unexplained infertility and borderline semen [J].

Aboulghar, MA ;

Mansour, RT ;

Serour, GI ;

Sattar, MA ;

Amin, YM .

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1996, 13 (01) :38-42

[2] Activation of human oocytes using calcium ionophore after ICSI increases fertilization and number of embryos for couples with low ICSI fertilization rates [J].

Adams, CA ;

Anderson, LS ;

Wood, SH .

HUMAN REPRODUCTION, 1999, 14 :32-32

[3] Failure of oocyte activation after intracytoplasmic sperm injection using round-headed sperm [J].

Battaglia, DE ;

Koehler, JK ;

Klein, NA ;

Tucker, MJ .

FERTILITY AND STERILITY, 1997, 68 (01) :118-122

[4] Fertilization failures and abnormal fertilization after intracytoplasmic sperm injection [J].

Flaherty, SP ;

Payne, D ;

Matthews, CD .

HUMAN REPRODUCTION, 1998, 13 :155-164

[5] In vitro fertilization-intracytoplasmic sperm injection split: an insemination method to prevent fertilization failure [J].

Hershlag, A ;

Paine, T ;

Kvapil, G ;

Feng, HL ;

Napolitano, B .

FERTILITY AND STERILITY, 2002, 77 (02) :229-232

[6] Successful pregnancy and delivery from frozen-thawed embryos after intracytoplasmic sperm injection using round-headed spermatozoa and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome [J].

Kim, ST ;

Cha, YB ;

Park, JM ;

Gye, MC .

FERTILITY AND STERILITY, 2001, 75 (02) :445-447

[7] SUCCESSFUL FERTILIZATION AND ESTABLISHMENT OF PREGNANCIES AFTER INTRACYTOPLASMIC SPERM INJECTION IN PATIENTS WITH GLOBOZOOSPERMIA [J].

LIU, J ;

NAGY, Z ;

JORIS, H ;

TOURNAYE, H ;

DEVROEY, P ;

VANSTEIRTEGHEM, A .

HUMAN REPRODUCTION, 1995, 10 (03) :626-629

[8] Calcium oscillations in mammalian eggs triggered by a soluble sperm protein [J].

Parrington, J ;

Swann, K ;

Shevchenko, VI ;

Sesay, AK ;

Lai, FA .

NATURE, 1996, 379 (6563) :364-368

[9] Sperm ultramorphology as a pathophysiological indicator of spermatogenesis in males suffering from varicocele [J].

Reichart, M ;

Eltes, F ;

Soffer, Y ;

Zigenreich, E ;

Yogev, L ;

Bartoov, B .

ANDROLOGIA, 2000, 32 (03) :139-145

[10] The role of in vitro fertilization and intracytoplasmic sperm injection in couples with unexplained infertility after failed intrauterine insemination [J].

Ruiz, A ;

Remohi, J ;

Minguez, Y ;

Guanes, PP ;

Simon, C ;

Pellicer, A .

FERTILITY AND STERILITY, 1997, 68 (01) :171-173

← 1 2 →