Expression of vasoactive intestinal peptide (VIP) receptors in human uterus

被引：24
作者：
Bajo, AM
Juarranz, MG
Valenzuela, P
Martínez, P
Prieto, JC
Guijarro, LG [1 ]
机构：
[1] Univ Alcala de Henares, Dept Bioquim & Biol Mol, Unidad Neuroendocrinol Mol, E-28871 Alcala De Henares, Spain
[2] Hosp Principe Asturias, Serv Obstet & Ginecol, E-28871 Alcala De Henares, Spain
[3] Hosp Principe Asturias, Serv Anat Patol, E-28871 Alcala De Henares, Spain
来源：
PEPTIDES | 2000年 / 21卷 / 09期
关键词：
VIP receptors; adenylyl cyclase; myometrium; endometrium; leiomyoma; Fallopian tube;
D O I：
10.1016/S0196-9781(00)00282-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We show the existence of functional vasoactive intestinal peptide (VIP) receptors in normal human female genital tract (endometrium, myometrium, ovary and Fallopian tube) as well as in leiomyoma (a frequent uterine pathology). The correlation between VIP binding and stimulation of adenylyl cyclase activity for all studied tissues was linear (r = 0.86) suggesting the expression of VIP receptors throughout the human female genital tract. Immunodetection of VIP receptor subtypes gave different molecular weights for VPAC(1) (47 kDa primarily) and VPAC(2) (65 kDa), which may be due to different glycosylation extents. In conclusion, this study demonstrates the expression of both subtypes of VIP receptors and their functionality in human female genital tract, suggesting that this neuropeptide could play an important physiological and pathophysiological role at this level. (C) 2000 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：1383 / 1388
页数：6
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