Tissue-specific alternative splicing of mouse brain type ryanodine receptor/calcium release channel mRNA

被引：32

作者：

Miyatake, R ^{[1
]}

Furukawa, A ^{[1
]}

Matsushita, M ^{[1
]}

Iwahashi, K ^{[1
]}

Nakamura, K ^{[1
]}

Ichikawa, Y ^{[1
]}

Suwaki, H ^{[1
]}

机构：

[1] KAGAWA MED UNIV,DEPT BIOCHEM,MIKI,KAGAWA 76107,JAPAN

来源：

FEBS LETTERS | 1996年 / 395卷 / 2-3期

关键词：

ryanodine receptor; calcium release channel; mouse brain; alternative splicing; RT-PCR;

D O I：

10.1016/0014-5793(96)01022-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We detected alternative splicing of the mouse brain type ryanodine receptor (RyR3) mRNA. The splicing variant,vas located in the transmembrane segment. The non-splicing type (RyR3-II) included a stretch of 341 bp, and that of the 13th codon was stop codon TAA. Reverse transcription-polymerase chain reaction (RT-PCR) analysis shows that RyR3-II mRNA was expressed in various peripheral tissues and brain at all developmental stages. However, interestingly, the splicing type (RyR3-I) mRNA was detected only in the cerebrum. These findings suggest that the splicing variants RyR3-I and RyR3-II may generate functional differences of RyR3 in a tissue-specific manner.

引用

页码：123 / 126

页数：4

共 50 条

[41] Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner
Watabe, Eichi
Ono, Shoichiro
Kuroyanagi, Hidehito
CYTOSKELETON, 2018, 75 (10) : 427 - 436
[42] Tissue-specific alternative splicing in the human INK4a/ARF cell cycle regulatory locus
Keith D Robertson
Peter A Jones
Oncogene, 1999, 18 : 3810 - 3820
[43] Tissue-specific alternative splicing in the human INK4a/ARF cell cycle regulatory locus
Robertson, KD
Jones, PA
ONCOGENE, 1999, 18 (26) : 3810 - 3820
[44] Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
Guo, Xuan
Zhao, Yiqing
Nguyen, Hieu
Liu, Tonghua
Wang, Zhenghe
Lou, Hua
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2018, (138):
[45] Ankyrin-G in skeletal muscle: Tissue-specific alternative splicing contributes to the complexity of the sarcolemmal cytoskeleton
Hopitzan, AA
Baines, AJ
Ludosky, MA
Recouvreur, M
Kordeli, E
EXPERIMENTAL CELL RESEARCH, 2005, 309 (01) : 86 - 98
[46] Modulation of the calmodulin-induced inhibition of sarcoplasmic reticulum calcium release channel (ryanodine receptor) by sulfhydryl oxidation in single channel current recordings and [3H]ryanodine binding
Suko, J
Hellmann, G
Drobny, H
JOURNAL OF MEMBRANE BIOLOGY, 2000, 174 (02) : 105 - 120
[47] Tissue-specific alternative splicing of rat brain fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase
Watanabe, F
Furuya, E
FEBS LETTERS, 1999, 458 (03) : 304 - 308
[48] Rbfox proteins regulate tissue-specific alternative splicing of Mef2D required for muscle differentiation
Runfola, Valeria
Sebastian, Soji
Dilworth, F. Jeffrey
Gabellini, Davide
JOURNAL OF CELL SCIENCE, 2015, 128 (04) : 631 - 637
[49] Brief Report: Alternative Splicing of Extra Domain A (EIIIA) of Fibronectin Plays a Tissue-Specific Role in Hematopoietic Homeostasis
Malara, Alessandro
Gruppi, Cristian
Celesti, Giuseppe
Romano, Bina
Laghi, Luigi
De Marco, Luigi
Muro, Andres S. F.
Balduini, Alessandra
STEM CELLS, 2016, 34 (08) : 2263 - 2268
[50] Tissue-specific expression of IL-15RA alternative splicing transcripts and its regulation by DNA methylation
Diniz, Susana N.
Pendeloski, Karen P. T.
Morgun, Andrey
Chepelev, Iouri
Gerbase-DeLima, Maria
Shulzhenko, Natalia
EUROPEAN CYTOKINE NETWORK, 2010, 21 (04) : 308 - 318

← 1 2 3 4 5 →