Tissue-specific alternative splicing of mouse brain type ryanodine receptor/calcium release channel mRNA

被引：32
|
作者：
Miyatake, R [1 ]
Furukawa, A [1 ]
Matsushita, M [1 ]
Iwahashi, K [1 ]
Nakamura, K [1 ]
Ichikawa, Y [1 ]
Suwaki, H [1 ]
机构：
[1] KAGAWA MED UNIV,DEPT BIOCHEM,MIKI,KAGAWA 76107,JAPAN
来源：
FEBS LETTERS | 1996年 / 395卷 / 2-3期
关键词：
ryanodine receptor; calcium release channel; mouse brain; alternative splicing; RT-PCR;
D O I：
10.1016/0014-5793(96)01022-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We detected alternative splicing of the mouse brain type ryanodine receptor (RyR3) mRNA. The splicing variant,vas located in the transmembrane segment. The non-splicing type (RyR3-II) included a stretch of 341 bp, and that of the 13th codon was stop codon TAA. Reverse transcription-polymerase chain reaction (RT-PCR) analysis shows that RyR3-II mRNA was expressed in various peripheral tissues and brain at all developmental stages. However, interestingly, the splicing type (RyR3-I) mRNA was detected only in the cerebrum. These findings suggest that the splicing variants RyR3-I and RyR3-II may generate functional differences of RyR3 in a tissue-specific manner.
引用
收藏
页码：123 / 126
页数：4
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