Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells

被引：18

作者：

Shih, Yung-Luen ^{[1
,2
,3
]}

Au, Man-Kuan ^{[4
]}

Liu, Ko-Lin ^{[1
]}

Yeh, Ming-Yang ^{[5
]}

Lee, Ching-Hsiao ^{[6
]}

Lee, Mei-Hui ^{[7
]}

Lu, Hsu-Feng ^{[8
,9
]}

Yang, Jiun-Long ^{[10
]}

Wu, Rick Sai-Chuen ^{[11
]}

Chung, Jing-Gung ^{[12
,13
]}

机构：

[1] Shin Kong Wu Ho Su Mem Hosp, Dept Pathol & Lab Med, Taipei, Taiwan

[2] Taipei Med Univ, Sch Med Lab Sci & Biotechnol, Taipei, Taiwan

[3] Fu Jen Catholic Univ, Sch Med, Coll Med, New Taipei, Taiwan

[4] Cheng Hsin Gen Hosp, Dept Orthoped, Taipei, Taiwan

[5] Cheng Hsin Gen Hosp, Off Director, Taipei, Taiwan

[6] Jen Teh Jr Coll Med Nursing & Management, Dept Med Technol, Houlong, Miaoli County, Taiwan

[7] Changhua Christian Hosp, Dept Genet Counseling Ctr, Changhua, Taiwan

[8] Fu Jen Catholic Univ, Restaurant Hotel & Inst Management, New Taipei, Taiwan

[9] Cheng Hsin Gen Hosp, Dept Clin Pathol, 45 Cheng Hsin St, Taipei 112, Taiwan

[10] China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc, Taichung, Taiwan

[11] China Med Univ, Sch Med, Taichung, Taiwan

[12] China Med Univ, Dept Biol Sci & Technol, 91 Hsueh Shih Rd, Taichung 40402, Taiwan

[13] Asia Univ, Dept Biotechnol, Taichung, Taiwan

来源：

ENVIRONMENTAL TOXICOLOGY | 2017年 / 32卷 / 11期

关键词：

migration and invasion; MMP-2; ouabain; TGFB1; 2; and; 3; U-2 OS cells; MATRIX METALLOPROTEINASES; IN-VITRO; CANCER CELLS; E-CADHERIN; INHIBITION; METASTASIS; SUPPRESSES; ADHESION; PATHWAY; GROWTH;

D O I：

10.1002/tox.22453

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Ouabain, the specific Na+/K+-ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 M, thus, we selected 0.25-1.0 M for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

引用

页码：2400 / 2413

页数：14

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