Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells

被引：12
|
作者：
Hong, Jong-Ah [1 ]
Choi, Na-Eun [1 ]
La, Yeo-Kyoung [1 ]
Nam, Ho Yeon [2 ]
Seo, Jiwon [2 ]
Lee, Jiyoun [1 ]
机构：
[1] Sungshin Univ, Dept Global Med Sci, Seoul 01133, South Korea
[2] Gwangju Inst Sci & Technol, Dept Chem, Gwangju 61005, South Korea
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2017年 / 15卷 / 38期
基金：
新加坡国家研究基金会;
关键词：
ALZHEIMERS-DISEASE; LEGUMAIN; CANCER; PROTEIN; EXPRESSION; DESIGN; TAU;
D O I：
10.1039/c7ob01467h
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
We developed a smart activity-based probe that detects the activity of asparaginyl endopeptidase (AEP) in live cells to monitor the dynamics of enzyme regulation. The newly designed probe generated a turn-on fluorescence signal in response to the activity of AEP in living cells without compromising the labelling efficiency or selectivity. Our probe closely reflected the enzyme activity in its native state, detecting subcellular AEP activity in colon cancer cells and neuronal cells.
引用
收藏
页码：8018 / 8022
页数：5
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