Photoswitchable Water-Soluble Quantum Dots: pcFRET Based on Amphiphilic Photochromic Polymer Coating

被引：111
作者：
Diaz, Sebastian A. [1 ]
Menendez, Guillermo O. [1 ]
Etchehon, Maria H. [1 ]
Giordano, Luciana [2 ]
Jovin, Thomas M. [2 ]
Jares-Erijman, Elizabeth A. [1 ]
机构：
[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, CIHIDECAR,CONICET, RA-1428 Buenos Aires, DF, Argentina
[2] Max Planck Inst Biophys Chem, Lab Cellular Dynam, D-37077 Gottingen, Germany
来源：
ACS NANO | 2011年 / 5卷 / 04期
关键词：
FRET; quantum dots; photochromism; amphiphilic polymer; nanoparticle coating; IMAGING MICROSCOPY; LIVE-CELL; FRET; DIARYLETHENES; PROBES; DIHETEROARYLETHENES; PHOTOLUMINESCENCE; NANOPARTICLES; MODULATION; LIFETIME;
D O I：
10.1021/nn103243c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel surface architecture was developed to generate biocompatible and stable photoswitchable quantum dots (psQDs). Photochromic diheteroarylethenes, which undergo thermally stable photoconversions between two forms with different spectral properties in organic solvents, were covalently linked to an amphiphilic polymer that self-assemble; with the lipophilic chains surrounding commercial hydrophobic core-shell CdSe/ZnS QDs. This strategy creates a small (similar to 7 nm diameter) nanoparticle (NP) that is soluble in aqueous medium. The NP retains and even enhances the desirable properties of the original QD (broad excitation, narrow emission, photostability), but the brightness of its emission can be tailored by light. The modulation of emission monitored by steady-state and time-resolved fluorescence was 35-40%. The psQDs exhibit unprecedented photostability and fatigue resistance over at least 16 cycles of photoconversion.
引用
收藏
页码：2795 / 2805
页数：11
相关论文
共 51 条
[21]   Imaging quantum dots switched on and off by photochromic fluorescence resonance energy transfer (pcFRET) [J].
Jares-Erijman, E ;
Giordano, L ;
Spagnuolo, C ;
Lidke, K ;
Jovin, TM .
MOLECULAR CRYSTALS AND LIQUID CRYSTALS, 2005, 430 :257-265
[22]   FRET imaging [J].
Jares-Erijman, EA ;
Jovin, TM .
NATURE BIOTECHNOLOGY, 2003, 21 (11) :1387-1395
[23]   FRET Imaging by kt/kf [J].
Julia Roberti, M. ;
Giordano, Luciana ;
Jovin, Thomas M. ;
Jares-Erijman, Elizabeth A. .
CHEMPHYSCHEM, 2011, 12 (03) :563-566
[24]   Novel photochromic conducting polymer having diarylethene derivative in the main chain [J].
Kawai, T ;
Kunitake, T ;
Irie, M .
CHEMISTRY LETTERS, 1999, (09) :905-906
[25]   Diarylethenes with intramolecular donor-acceptor structures for photo-induced electrochemical change [J].
Kim, Eunkyoung ;
Kim, Miyoung ;
Kim, Kyongtae .
TETRAHEDRON, 2006, 62 (29) :6814-6821
[26]   Fluorescence lifetime and polarization-resolved imaging in cell biology [J].
Levitt, James A. ;
Matthews, Daniel R. ;
Ameer-Beg, Simon M. ;
Suhling, Klaus .
CURRENT OPINION IN BIOTECHNOLOGY, 2009, 20 (01) :28-36
[27]   Design of an amphiphilic polymer for nanoparticle coating and functionalization [J].
Lin, Cheng-An J. ;
Sperling, Ralph A. ;
Li, Jimmy K. ;
Yang, Ting-Ya ;
Li, Pei-Yun ;
Zanella, Marco ;
Chang, Walter H. ;
Parak, Wolfgang J. .
SMALL, 2008, 4 (03) :334-341
[28]   Optical lock-in detection imaging microscopy for contrast-enhanced imaging in living cells [J].
Marriott, Gerard ;
Mao, Shu ;
Sakata, Tomoyo ;
Ran, Jing ;
Jackson, David K. ;
Petchprayoon, Chutima ;
Gomez, Timothy J. ;
Warp, Erica ;
Tulyathan, Orapim ;
Aaron, Holly L. ;
Isacoff, Ehud Y. ;
Yan, Yuling .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (46) :17789-17794
[29]   Quantum dot-based resonance energy transfer and its growing application in biology [J].
Medintz, Igor L. ;
Mattoussi, Hedi .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2009, 11 (01) :17-45
[30]   Reversible modulation of quantum dot photoluminescence using a protein-bound photochromic fluorescence resonance energy transfer acceptor [J].
Medintz, IL ;
Trammell, SA ;
Mattoussi, H ;
Mauro, JM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (01) :30-31
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