Enzyme-free amplified SERS immunoassay for the ultrasensitive detection of disease biomarkers

被引：21
|
作者：
Zhang, Xuan-Xuan [1 ,2 ]
Xu, Duo [1 ,2 ]
Guo, Dan [1 ,2 ]
Han, Huan-Xing [3 ]
Li, Da-Wei [1 ,2 ]
Ma, Wei [1 ,2 ]
机构：
[1] East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China
[2] East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China
[3] Aliex Technol Grp Co Ltd, 152,Lane 468,North Hengshahe Rd, Shanghai 201108, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 19期
基金：
中国国家自然科学基金;
关键词：
CANCER BIOMARKER; ELECTROCHEMICAL DETECTION; NANOPARTICLES; GRAPHENE;
D O I：
10.1039/c9cc09379f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We developed a novel enzyme-free amplified SERS immunoassay by combining silver nanoparticle (AgNP)-linked immunoreaction and SERS transduction for the detection of disease biomarkers. As a proof of concept, our method was successfully illustrated with the disease biomarker alpha-fetoprotein with the detection limit of 3.3 x 10(-13) g mL(-1) and a double-blind experiment consisting of tens of serum samples was performed to confirm its reliability.
引用
收藏
页码：2933 / 2936
页数：4
相关论文
共 50 条
  • [11] Mercury detection based on label-free and isothermal enzyme-free amplified fluorescence platform
    Meng, Fanbin
    Xu, Hui
    Yao, Xue
    Qin, Xuan
    Jiang, Tingting
    Gao, Shanmin
    Zhang, Yahui
    Yang, Di
    Liu, Xia
    TALANTA, 2017, 162 : 368 - 373
  • [12] Enzyme-nanozyme cascade-amplified ratiometric fluorescence immunoassay for the ultrasensitive detection of saxitoxin
    Luo, Lin
    Jia, Bao-Zhu
    Wei, Xiao-Qun
    Wang, Xing-Xing
    Wang, Bing-Zhi
    Wang, Hong
    Lei, Hong-Tao
    Xu, Zhen-Lin
    SENSORS AND ACTUATORS B-CHEMICAL, 2023, 393
  • [13] Droplet-free and enzyme-free digital immunoassay based on fluorescent microspheres for protein detection
    Xie, Yaping
    Li, Huiji
    Tang, Yao
    Lian, Xiaohuan
    Dai, Lizhong
    Tian, Songbai
    SENSORS AND ACTUATORS B-CHEMICAL, 2023, 396
  • [14] FeMoO4 based, enzyme-free electrochemical biosensor for ultrasensitive detection of norepinephrine
    Samdani, Kunda J.
    Samdani, Jitendra S.
    Kim, Nam Hoon
    Lee, Joong Hee
    BIOSENSORS & BIOELECTRONICS, 2016, 81 : 445 - 453
  • [15] Sensitive SERS detection of miRNA via enzyme-free DNA machine signal amplification
    Li, Xiaoxiao
    Ye, Sujuan
    Luo, Xiliang
    CHEMICAL COMMUNICATIONS, 2016, 52 (67) : 10269 - 10272
  • [16] A simple enzyme-free SERS sensor for the rapid and sensitive detection of hydrogen peroxide in food
    Li, Yangyang
    Wang, Yuqiu
    Fu, Cuicui
    Wu, Yan
    Cao, Haiyan
    Shi, Wenbing
    Jung, Young Mee
    ANALYST, 2020, 145 (02) : 607 - 612
  • [17] Ultrasensitive detection of multiple Alzheimer's disease biomarkers by SERS-LFA
    Zhan, Yuanbao
    Fei, Ruihua
    Lu, Yu
    Wan, Yu
    Wu, Xuming
    Dong, Jian
    Meng, Dianhuai
    Ge, Qinyu
    Zhao, Xiangwei
    ANALYST, 2022, 147 (18) : 4124 - 4131
  • [18] Enzyme-Free Ultrasensitive Electrochemical Immunoassay Based on the Strategy of Converting Titanium Phosphate Nanospheres into Redox Active Molybdophosphate
    Tan, Xiaofeng
    Zhang, Lianhua
    Li, Huaigu
    Liu, Siquan
    Miao, Luyang
    Li, He
    Zheng, Gengxiu
    JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2017, 13 (08) : 973 - 979
  • [19] A New Enzyme-Free Electrochemical Immunoassay for Escherichia coli Detection using Magnetic Nanoparticles
    Kozitsina, A.
    Svalova, T.
    Malysheva, N.
    Glazyrina, Y.
    Matern, A.
    ANALYTICAL LETTERS, 2016, 49 (02) : 245 - 257
  • [20] Enzyme-free immunoassay for rapid, sensitive, and selective detection of C-reactive protein
    Munusamy, Sathishkumar
    Zheng, Haiyan
    Jahani, Rana
    Zhou, Shuo
    Chen, Jun
    Kong, Juanhua
    Guan, Xiyun
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2024, 416 (29) : 6985 - 6994
12345