Enzyme-free amplified SERS immunoassay for the ultrasensitive detection of disease biomarkers

被引：21
|
作者：
Zhang, Xuan-Xuan [1 ,2 ]
Xu, Duo [1 ,2 ]
Guo, Dan [1 ,2 ]
Han, Huan-Xing [3 ]
Li, Da-Wei [1 ,2 ]
Ma, Wei [1 ,2 ]
机构：
[1] East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China
[2] East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China
[3] Aliex Technol Grp Co Ltd, 152,Lane 468,North Hengshahe Rd, Shanghai 201108, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 19期
基金：
中国国家自然科学基金;
关键词：
CANCER BIOMARKER; ELECTROCHEMICAL DETECTION; NANOPARTICLES; GRAPHENE;
D O I：
10.1039/c9cc09379f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We developed a novel enzyme-free amplified SERS immunoassay by combining silver nanoparticle (AgNP)-linked immunoreaction and SERS transduction for the detection of disease biomarkers. As a proof of concept, our method was successfully illustrated with the disease biomarker alpha-fetoprotein with the detection limit of 3.3 x 10(-13) g mL(-1) and a double-blind experiment consisting of tens of serum samples was performed to confirm its reliability.
引用
收藏
页码：2933 / 2936
页数：4
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