3D VISUALIZATION OF HIV VIRIONS BY CRYOELECTRON TOMOGRAPHY

被引：24

作者：

Liu, Jun ^{[1
]}

Wright, Elizabeth R. ^{[2
]}

Winkler, Hanspeter ^{[3
]}

机构：

[1] Univ Texas Houston, Sch Med, Dept Pathol & Lab Med, Houston, TX 77030 USA

[2] Emory Univ, Sch Med, Dept Pediat, Atlanta, GA USA

[3] Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32306 USA

来源：

METHODS IN ENZYMOLOGY, VOL 483: CRYO-EM, PART C: ANALYSES, INTERPRETATION, AND CASE STUDIES | 2010年 / 483卷

关键词：

3-DIMENSIONAL STRUCTURE; ELECTRON TOMOGRAPHY; GAG PROTEIN; INTERSUBUNIT INTERACTIONS; ENVELOPE GLYCOPROTEIN; TILT-SERIES; VIRUS; MICROSCOPY; REVEALS; GP120;

D O I：

10.1016/S0076-6879(10)83014-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the human immunodeficiency virus (HIV) and some of its components have been difficult to study in three-dimensions (3D) primarily because of their intrinsic structural variability. Recent advances in cryoelectron tomography (cryo-ET) have provided a new approach for determining the 3D structures of the intact virus, the HIV capsid, and the envelope glycoproteins located on the viral surface. A number of cryo-ET procedures related to specimen preservation, data collection, and image processing are presented in this chapter. The techniques described herein are well suited for determining the ultrastructure of bacterial and viral pathogens and their associated molecular machines in situ at nanometer resolution.

引用

页码：267 / 290

页数：24

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