Analysis of amino acids in cell culture supernatant using capillary electrophoresis with contactless conductivity detection

被引：9
作者：
Mikkonen, Saara [1 ]
Emmer, Asa [1 ]
机构：
[1] KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, Div Appl Phys Chem, Teknikringen 36, SE-10044 Stockholm, Sweden
来源：
ELECTROPHORESIS | 2021年 / 42卷 / 19期
基金：
欧盟地平线“2020”;
关键词：
Amino acid; Capillary electrophoresis; Cell culture; Contactless conductivity;
D O I：
10.1002/elps.202100122
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
CE-(CD)-D-4 methods for the analysis of amino acids (AAs) are presented. Combining the results from two methods with acetic acid and cyclodextrin-based BGEs, 20 proteinogenic AAs could be analyzed using CE. CE-(CD)-D-4 was also, for the first time, applied to analyze free AAs in samples of mammalian cell culture supernatant. After dilution as only sample preparation, combining the results of the two CE methods allowed monitoring the concentration changes of 17 AAs in samples taken during the cultivation of CHO cells.
引用
收藏
页码：1924 / 1927
页数：4
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