Defining a mesenchymal progenitor niche at single-cell resolution

被引：103
作者：
Kumar, Maya E. [1 ,2 ]
Bogard, Patrick E. [1 ,2 ]
Espinoza, F. Hernan [1 ,2 ]
Menke, Douglas B. [1 ,3 ,4 ]
Kingsley, David M. [1 ,4 ]
Krasnow, Mark A. [1 ,2 ]
机构：
[1] Stanford Univ, Sch Med, Howard Hughes Med Inst, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
[3] Univ Georgia, Dept Genet, Athens, GA 30602 USA
[4] Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA
来源：
SCIENCE | 2014年 / 346卷 / 6211期
关键词：
SMOOTH-MUSCLE; STEM-CELLS; INTESTINAL CRYPT; LUNG DEVELOPMENT; SELF-RENEWAL; POPULATIONS; HOMEOSTASIS; CONTRIBUTE; PROGRAM; GENE;
D O I：
10.1126/science.1258810
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Most vertebrate organs are composed of epithelium surrounded by support and stromal tissues formed from mesenchyme cells, which are not generally thought to form organized progenitor pools. Here, we use clonal cell labeling with multicolor reporters to characterize individual mesenchymal progenitors in the developing mouse lung. We observe a diversity of mesenchymal progenitor populations with different locations, movements, and lineage boundaries. Airway smooth muscle (ASM) progenitors map exclusively to mesenchyme ahead of budding airways. Progenitors recruited from these tip pools differentiate into ASM around airway stalks; flanking stalk mesenchyme can be induced to form an ASM niche by a lateral bud or by an airway tip plus focal Wnt signal. Thus, mesenchymal progenitors can be organized into localized and carefully controlled domains that rival epithelial progenitor niches in regulatory sophistication.
引用
收藏
页码：827 / +
页数：10
相关论文
共 40 条
[11]   Levels of mesenchymal FGFR2 signaling modulate smooth muscle progenitor cell commitment in the lung [J].
De langhe, Stijn P. ;
Carraro, Gianni ;
Warburton, David ;
Hajihosseini, Mohammad K. ;
Bellusci, Saverio .
DEVELOPMENTAL BIOLOGY, 2006, 299 (01) :52-62
[12]   Alveolar progenitor and stem cells in lung development, renewal and cancer [J].
Desai, Tushar J. ;
Brownfield, Douglas G. ;
Krasnow, Mark A. .
NATURE, 2014, 507 (7491) :190-+
[13]   Derivation of lung mesenchymal lineages from the fetal mesothelium requires hedgehog signaling for mesothelial cell entry [J].
Dixit, Radhika ;
Ai, Xingbin ;
Fine, Alan .
DEVELOPMENT, 2013, 140 (21) :4398-4406
[14]  
Flint JM, 1906, AM J ANAT, V6, P1
[15]   Wnt2 signaling is necessary and sufficient to activate the airway smooth muscle program in the lung by regulating myocardin/Mrtf-B and Fgf10 expression [J].
Goss, Ashley M. ;
Tian, Ying ;
Cheng, Lan ;
Yang, Jifu ;
Zhou, Diane ;
Cohen, Ethan David ;
Morrisey, Edward E. .
DEVELOPMENTAL BIOLOGY, 2011, 356 (02) :541-552
[16]   Radial Construction of an Arterial Wall [J].
Greif, Daniel M. ;
Kumar, Maya ;
Lighthouse, Janet K. ;
Hum, Justine ;
An, Andrew ;
Ding, Ling ;
Red-Horse, Kristy ;
Espinoza, F. Heman ;
Olson, Lorin ;
Offermanns, Stefan ;
Krasnow, Mark A. .
DEVELOPMENTAL CELL, 2012, 23 (03) :482-493
[17]   INDUCTIVE EPITHELIO-MESENCHYMAL INTERACTION IN CULTURED ORGAN RUDIMENTS OF THE MOUSE [J].
GROBSTEIN, C .
SCIENCE, 1953, 118 (3054) :52-55
[18]   Fate Tracing Reveals the Pericyte and Not Epithelial Origin of Myofibroblasts in Kidney Fibrosis [J].
Humphreys, Benjamin D. ;
Lin, Shuei-Liong ;
Kobayashi, Akio ;
Hudson, Thomas E. ;
Nowlin, Brian T. ;
Bonventre, Joseph V. ;
Valerius, M. Todd ;
McMahon, Andrew P. ;
Duffield, Jeremy S. .
AMERICAN JOURNAL OF PATHOLOGY, 2010, 176 (01) :85-97
[19]  
MacCord K., 2012, EMBRYO PROJECT ENCYC
[20]   Fgf10 expression identifies parabronchial smooth muscle cell progenitors and is required for their entry into the smooth muscle cell lineage [J].
Mailleux, AA ;
Kelly, R ;
Veltmaat, JM ;
De Langhe, SP ;
Zalfran, S ;
Thiery, JP ;
Bellusci, S .
DEVELOPMENT, 2005, 132 (09) :2157-2166
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