Comparison of the cytogenotoxicity induced by five different dental alloys using four in vitro assays

被引：7
作者：
Chen Zhihong [1 ,2 ]
Lu Yezhen [1 ]
Gu Zhiyuan [3 ]
Yang Zhongqiao [4 ]
Li Xiaoxue [1 ]
Ryan Yifeng [2 ]
Fang Weikun [2 ]
He Jiliang [1 ]
机构：
[1] Zhejiang Univ, Coll Med, Inst Environm Med, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Med, Stomatol Hosp, Hangzhou 310006, Zhejiang, Peoples R China
[3] Zhejiang Chinese Med Univ, Coll Stomotol, Hangzhou 310053, Zhejiang, Peoples R China
[4] Hangzhou Ctr Dis Control & Prevent, Hangzhou 310021, Zhejiang, Peoples R China
来源：
DENTAL MATERIALS JOURNAL | 2011年 / 30卷 / 06期
关键词：
Alloys; Neutral red uptake assay; CCK-8; assay; Micronucleus assay; Comet assay; CASTING ALLOYS; NICKEL; CYTOTOXICITY; GENOTOXICITY; CORROSION; CELLS; CARCINOGENICITY; CHROMIUM;
D O I：
10.4012/dmj.2011-127
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The purpose of present investigation was to compare the cyto-genotoxicity induced by five dental alloys in vitro. The cyto-genotoxicity induced by five dental alloy extracts on human B lymphoblast cells was assessed with neutral red uptake (NRU), CCK-8, comet and micronucleus (CBMN) assays in vitro, respectively. The results of in vitro comet and CBMN assays indicated that DNA damage (% tail DNA) and micronucleus frequencies (MNFs) in all exposure groups did not significantly increase, as compared with the control group. However, the results of NRU and CCK-8 assays demonstrated that there were to some extent differences in the cytotoxicity among 5 dental alloy extracts in vitro. The cytotoxicity may be relevant to the Ni and Be ions released in the alloy extract.
引用
收藏
页码：861 / 868
页数：8
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