Endothelial f-actin depolymerization enables leukocyte transmigration

被引:20
作者
Isac, Laura [2 ,3 ]
Thoelking, Gerold [2 ,4 ]
Schwab, Albrecht [2 ]
Oberleithner, Hans [2 ]
Riethmuller, Christoph [1 ,2 ]
机构
[1] Serend Ip GmbH, Ctr Technol, D-48149 Munster, Germany
[2] Univ Munster, Inst Physiol 2, D-48149 Munster, Germany
[3] Univ Clin Bucharest, Rheumatol Unit, Bucharest 70709, Romania
[4] Univ Clin Munster, Med Clin D, D-48149 Munster, Germany
关键词
Leukocyte; Endothelium; Transmigration; Cell mechanics; Atomic force microscopy; TIRF; CELL-CELL CONTACTS; TRANSENDOTHELIAL MIGRATION; TRANSCELLULAR MIGRATION; VASCULAR ENDOTHELIUM; NEUTROPHIL ADHESION; TIGHT JUNCTIONS; IN-VITRO; ICAM-1; DIAPEDESIS; PHOSPHORYLATION;
D O I
10.1007/s00216-010-3978-z
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A demanding task of medicine is to understand and control the immune system. Central players in the cellular immune response are the leukocytes that leave the blood stream for host defense. Endothelial cells limit the emigration rate of leukocytes. Being located between blood and tissues, they permit or deny the passage. The exact mechanism of this process called diapedesis is not solved yet. Leukocytes can principally traverse either between cells (paracellularly) or directly through an individual endothelial cell (transcellularly). The transcellular way has recently gained experimental support, but it is not clear how the endothelial cytoskeleton manages to open and close a transmigratory channel. Atomic force microscopy was used to investigate the endothelial cytoskeleton. In order to directly access the leukocyte-endothelial interaction site, we applied a special protocol ("nanosurgery"). As a result, the endothelial cell turned out to become softer in a confined region strictly underneath the leukocyte. Fluorescence microscopy confirmed a depolymerization of the f-actin strands at the invasion site. Leukocytes dramatically rearrange the endothelial cytoskeleton to form transmigratory channels.
引用
收藏
页码:2351 / 2358
页数:8
相关论文
共 37 条
[1]  
ADAMI JG, 1909, INTRO STUDY PATHOLOG
[2]   ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration [J].
Allingham, Michael J. ;
van Buul, Jaap D. ;
Burridge, Keith .
JOURNAL OF IMMUNOLOGY, 2007, 179 (06) :4053-4064
[3]   Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes [J].
Barreiro, O ;
Yáñez-Mó, M ;
Serrador, JM ;
Montoya, MC ;
Vicente-Manzanares, M ;
Tejedor, R ;
Furthmayr, H ;
Sánchez-Madrid, F .
JOURNAL OF CELL BIOLOGY, 2002, 157 (07) :1233-1245
[4]  
Burns AR, 1997, J IMMUNOL, V159, P2893
[5]  
Canetta E, 2005, BIORHEOLOGY, V42, P321
[6]   Trans-cellular migration: cell-cell contacts get intimate [J].
Carman, Christopher V. ;
Springer, Timothy A. .
CURRENT OPINION IN CELL BIOLOGY, 2008, 20 (05) :533-540
[7]   Transcellular diapedesis is initiated by invasive podosomes [J].
Carman, Christopher V. ;
Sage, Peter T. ;
Sciuto, Tracey E. ;
de la Fuente, Miguel A. ;
Geha, Raif S. ;
Ochs, Hans D. ;
Dvorak, Harold F. ;
Dvorak, Ann M. ;
Springer, Timothy A. .
IMMUNITY, 2007, 26 (06) :784-797
[8]   A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them [J].
Carman, CV ;
Springer, TA .
JOURNAL OF CELL BIOLOGY, 2004, 167 (02) :377-388
[9]   Shear forces promote lymphocyte migration across vascular endothelium bearing apical chemokines [J].
Cinamon, G ;
Shinder, V ;
Alon, R .
NATURE IMMUNOLOGY, 2001, 2 (06) :515-522
[10]  
DUSTIN ML, 1989, COLD SPRING HARB SYM, V54, P753