Conditional gene knockout using Cre recombinase

被引：70

作者：

Le, YZ ^{[1
]}

Sauer, B ^{[1
]}

机构：

[1] Oklahoma Med Res Fdn, Dev Biol Program, Oklahoma City, OK 73104 USA

来源：

MOLECULAR BIOTECHNOLOGY | 2001年 / 17卷 / 03期

关键词：

Cre; site-specific recombination; knockout; FACS; ES cells; GFP; STOP cassette; fusion proteins; transgenic mice; loxP;

D O I：

10.1385/MB:17:3:269

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional alleles and on reliable detection of c rr gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged Cre protein.

引用

页码：269 / 275

页数：7

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