Conditional gene knockout using Cre recombinase

被引：68
作者：
Le, YZ [1 ]
Sauer, B [1 ]
机构：
[1] Oklahoma Med Res Fdn, Dev Biol Program, Oklahoma City, OK 73104 USA
来源：
MOLECULAR BIOTECHNOLOGY | 2001年 / 17卷 / 03期
关键词：
Cre; site-specific recombination; knockout; FACS; ES cells; GFP; STOP cassette; fusion proteins; transgenic mice; loxP;
D O I：
10.1385/MB:17:3:269
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre's ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional alleles and on reliable detection of c rr gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged Cre protein.
引用
收藏
页码：269 / 275
页数：7
相关论文
共 19 条
  • [1] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA
    CHEN, C
    OKAYAMA, H
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) : 2745 - 2752
  • [2] AN IN OUT STRATEGY USING GENE TARGETING AND FLP RECOMBINASE FOR THE FUNCTIONAL DISSECTION OF COMPLEX DNA REGULATORY ELEMENTS - ANALYSIS OF THE BETA-GLOBIN LOCUS-CONTROL REGION
    FIERING, S
    KIM, CG
    EPNER, EM
    GROUDINE, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (18) : 8469 - 8473
  • [3] Brief expression of a GFPcre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions
    Gagneten, S
    Le, YZ
    Miller, J
    Sauer, B
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (16) : 3326 - 3331
  • [4] GENDRONMAGUIRE M, 1993, METHOD ENZYMOL, V225, P794
  • [5] Gordon J W, 1993, Hum Cell, V6, P161
  • [6] DELETION OF A DNA-POLYMERASE-BETA GENE SEGMENT IN T-CELLS USING CELL-TYPE-SPECIFIC GENE TARGETING
    GU, H
    MARTH, JD
    ORBAN, PC
    MOSSMANN, H
    RAJEWSKY, K
    [J]. SCIENCE, 1994, 265 (5168) : 103 - 106
  • [7] Helm R., 1995, Nature, V373, P663
  • [8] Hoess R.H., 1990, NUCLEIC ACIDS MOL BI, V4, P99
  • [9] Efficient in vivo manipulation of mouse genomic sequences at the zygote stage
    Lakso, M
    Pichel, JG
    Gorman, JR
    Sauer, B
    Okamoto, Y
    Lee, E
    Alt, FW
    Westphal, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) : 5860 - 5865
  • [10] TARGETED ONCOGENE ACTIVATION BY SITE-SPECIFIC RECOMBINATION IN TRANSGENIC MICE
    LAKSO, M
    SAUER, B
    MOSINGER, B
    LEE, EJ
    MANNING, RW
    YU, SH
    MULDER, KL
    WESTPHAL, H
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) : 6232 - 6236
12