Internal benchmarking of a human blood-brain barrier cell model for screening of nanoparticle uptake and transcytosis

被引:69
作者
Ragnaill, Michelle Nic [1 ,2 ]
Brown, Meredith [2 ,3 ]
Ye, Dong [2 ]
Bramini, Mattia [2 ]
Callanan, Sean [3 ]
Lynch, Iseult [2 ]
Dawson, Kenneth A. [2 ]
机构
[1] Univ Coll Dublin, Ctr BioNano Interact, Sch Chem & Chem Biol, Dublin 4, Ireland
[2] Univ Coll Dublin, UCD Conway Inst, Dublin 4, Ireland
[3] Univ Coll Dublin, Sch Agr Food Sci & Vet Med, Dublin 4, Ireland
关键词
Blood-brain barrier; Nanoparticles; Transcytosis; Bionanointeraction; DRUG TRANSPORT; CORONA; LINE;
D O I
10.1016/j.ejpb.2010.12.024
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Transport of drugs across the blood-brain barrier, which protects the brain from harmful agents, is considered the holy grail of targeted delivery, due to the extreme effectiveness of this barrier at preventing passage of non-essential molecules through to the brain. This has caused severe limitations for therapeutics for many brain-associated diseases, such as HIV and neurodegenerative diseases. Nanomaterials, as a result of their small size (in the order of many protein-lipid clusters routinely transported by cells) and their large surface area (which acts as a scaffold for proteins thereby rendering nanoparticles as biological entities) offer great promise for neuro-therapeutics. However, in parallel with developing neuro-therapeutic applications based on nanotechnology, it is essential to ensure their safety and long-term consequences upon reaching the brain. One approach to determining safe application of nanomaterials in biology is to obtain a deep mechanistic understanding of the interactions between nanomaterials and living systems (bionanointeractions). To this end, we report here on the establishment and internal round robin validation of a human cell model of the blood-brain barrier for use as a tool for screening nanoparticles interactions, and assessing the critical nanoscale parameters that determine transcytosis. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:360 / 367
页数:8
相关论文
共 20 条
[1]   Astrocyte-endothelial interactions at the blood-brain barrier [J].
Abbott, NJ ;
Rönnbäck, L ;
Hansson, E .
NATURE REVIEWS NEUROSCIENCE, 2006, 7 (01) :41-53
[2]   Multifunctional Nanocarriers for diagnostics, drug delivery and targeted treatment across blood-brain barrier: perspectives on tracking and neuroimaging [J].
Bhaskar, Sonu ;
Tian, Furong ;
Stoeger, Tobias ;
Kreyling, Wolfgang ;
de la Fuente, Jesus M. ;
Grazu, Valeria ;
Borm, Paul ;
Estrada, Giovani ;
Ntziachristos, Vasilis ;
Razansky, Daniel .
PARTICLE AND FIBRE TOXICOLOGY, 2010, 7
[3]   Detailed identification of plasma proteins adsorbed on copolymer nanoparticles [J].
Cedervall, Tommy ;
Lynch, Iseult ;
Foy, Martina ;
Berggard, Tord ;
Donnelly, Seamas C. ;
Cagney, Gerard ;
Linse, Sara ;
Dawson, Kenneth A. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (30) :5754-5756
[4]   Polyomavirus JC infects human brain microvascular endothelial cells independent of serotonin receptor 2A [J].
Chapagain, Moti L. ;
Verma, Saguna ;
Mercier, Frederic ;
Yanagihara, Richard ;
Nerurkar, Vivek R. .
VIROLOGY, 2007, 364 (01) :55-63
[5]   Blood-brain barrier tight junction disruption in human immunodeficiency virus-1 encephalitis [J].
Dallasta, LM ;
Pisarov, LA ;
Esplen, JE ;
Werley, JV ;
Moses, AV ;
Nelson, JA ;
Achim, CL .
AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (06) :1915-1927
[6]   The response of a co-culture lung model to fine and ultrafine particles of incinerator fly ash at the air-liquid interface [J].
Diabate, Silvia ;
Muelhopt, Sonja ;
Paur, Hanns-Rudolf ;
Krug, Harald F. .
ATLA-ALTERNATIVES TO LABORATORY ANIMALS, 2008, 36 (03) :285-298
[7]   Immunological properties of engineered nanomaterials [J].
Dobrovolskaia, Marina A. ;
Mcneil, Scott E. .
NATURE NANOTECHNOLOGY, 2007, 2 (08) :469-478
[8]   Growth and characterisation of a cell culture model of the feline blood-brain barrier [J].
Fletcher, NF ;
Brayden, DJ ;
Brankin, B ;
Worrall, S ;
Callanan, JJ .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2006, 109 (3-4) :233-244
[9]   Complete high-density lipoproteins in nanoparticle corona [J].
Hellstrand, Erik ;
Lynch, Iseult ;
Andersson, Astra ;
Drakenberg, Torbjorn ;
Dahlback, Bjorn ;
Dawson, Kenneth A. ;
Linse, Sara ;
Cedervall, Tommy .
FEBS JOURNAL, 2009, 276 (12) :3372-3381
[10]   The targeted delivery of cancer drugs across the blood-brain barrier: chemical modifications of drugs or drug-nanoparticles? [J].
Juillerat-Jeanneret, Ludenne .
DRUG DISCOVERY TODAY, 2008, 13 (23-24) :1099-1106