Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different Solanum tuberosum Varieties

被引：34

作者：

De Masi, Luigi ^{[1
]}

Bontempo, Paola ^{[2
]}

Rigano, Daniela ^{[3
]}

Stiuso, Paola ^{[2
]}

Carafa, Vincenzo ^{[2
]}

Nebbioso, Angela ^{[2
]}

Piacente, Sonia ^{[4
]}

Montoro, Paola ^{[4
]}

Aversano, Riccardo ^{[5
]}

D'Amelia, Vincenzo ^{[5
]}

Carputo, Domenico ^{[5
]}

Altucci, Lucia ^{[2
]}

机构：

[1] Natl Res Council CNR, Inst Biosci & Bioresources IBBR, Via Univ 133, I-80055 Naples, Italy

[2] Univ Campania Luigi Vanvitelli, Dept Precis Med, Via L De Crecchio 7, I-80138 Naples, Italy

[3] Univ Naples Federico II, Dept Pharm, Via Domen Montesano 49, I-80131 Naples, Italy

[4] Univ Salerno, Dept Pharm, Via Giovanni Paolo 2 132, I-84084 Salerno, Italy

[5] Univ Naples Federico II, Dept Agr Sci, Via Univ 100, I-80055 Naples, Italy

来源：

MOLECULES | 2020年 / 25卷 / 01期

关键词：

pigmented potatoes; potato tuber extract; leukemia cells; cell cycle modulation; bioactive molecules; potato genotyping; TRANSCRIPTION FACTORS; QUANTITATIVE-ANALYSIS; CELL-DIFFERENTIATION; POTATO VARIETIES; ANTIOXIDANT; ANTHOCYANINS; APOPTOSIS; AN1;

D O I：

10.3390/molecules25010233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants produce a vast array of biomolecules with beneficial effects for human health. In this study, polyphenol and anthocyanin-rich extracts (PAE) from pigmented tubers of Solanum tuberosum L. varieties "Blue Star", "Magenta Love", and "Double Fun" in comparison with the more extensively studied "Vitelotte" were evaluated and compared for antiproliferative effects in human leukemia cells, and their phytochemical and genetic profiles were determined. In U937 cells, upon treatment with PAE, it was possible to reveal the expression of specific apoptotic players, such as caspase 8, 9, 3, and poly (ADP-ribose) polymerase (PARP), as well as the induction of monocyte and granulocyte differentiation. A liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) investigation revealed the presence of polyphenolic compounds in all the varieties of potatoes analyzed, among which caffeoyl and feruloyl quinic acid derivatives were the most abundant, as well as several acylated anthocyanins. Each pigmented variety was genotyped by DNA-based molecular markers, and flavonoid-related transcription factors were profiled in tubers in order to better characterize these outstanding resources and contribute to their exploitation in breeding. Interesting biological activities were observed for "Blue Star" and "Vitelotte" varieties with respect to the minor or no effect of the "Double Fun" variety.

引用

页数：19

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