Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysate from unicorn leatherjacket skin

被引：52
作者：
Karnjanapratum, Supatra [2 ]
O'Callaghan, Yvonne C. [1 ]
Benjakul, Soottawat [2 ]
O'Brien, Nora [1 ]
机构：
[1] Natl Univ Ireland Univ Coll Cork, Sch Food & Nutr Sci, Cork, Ireland
[2] Prince Songkla Univ, Fac Agroind, Dept Food Technol, Hat Yai 90112, Songkhla, Thailand
来源：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2016年 / 96卷 / 09期
关键词：
gelatin hydrolysate; unicorn leatherjacket skin; antioxidant activity; immunomodulatory effect; antiproliferative activity; PROTEIN; ANTICANCER; PEPTIDES; CELLS; LIVER;
D O I：
10.1002/jsfa.7504
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
BACKGROUND: The in vitro cellular bioactivities including, antioxidant, immunomodulatory and antiproliferative effects of a gelatin hydrolysate (GH) prepared from unicorn leatherjacket skin, using partially purified glycyl endopeptidase, were investigated in order to optimize the use of fish skin waste products as functional food ingredients. RESULTS: GH under the tested concentrations (750-1500 mu g mL(-1)) protected against H2O2-induced DNA damage in U937 cells. GH also protected against the H2O2-induced reduction in cellular antioxidant enzyme activities, superoxide dismutase and catalase, in HepG2 cells. GH demonstrated immunomodulatory potential by reducing pro-inflammatory cytokine (interleukin-6 (IL-6) and IL-1 beta) production and nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Cell proliferation in human colon cancer (Caco-2) cells was significantly reduced in a dose-dependent manner following incubation with GH. CONCLUSION: These results indicate that GH has several bioactivities which support its potential as a promising functional food ingredient with various health benefits. (C) 2015 Society of Chemical Industry
引用
收藏
页码：3220 / 3226
页数：7
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