Cathodic stripping voltammetric determination of metallothioneins

被引：16
作者：
Scarano, G
Morelli, E
机构：
[1] Istituto di Biofisica, Consiglio Nazionale delle Ricerche, I-56127 Pisa, Via S. Lorenzo
来源：
ELECTROANALYSIS | 1996年 / 8卷 / 04期
关键词：
metallothioneins; cathodic stripping voltammetry; metal-binding proteins;
D O I：
10.1002/elan.1140080418
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A cathodic stripping voltammetric method for the determination of metallothioneins (MT) is proposed. The procedure is based on the controlled adsorption of the protein on the mercury electrode after addition of an excess of Cu-II to the solution. The effects of accumulation time, adsorption potential and copper concentration have been examined and the analytical procedure has been optimized. Using an accumulation time of 300 s, a MT concentration of 0.2 nM can be detected.
引用
收藏
页码：396 / 398
页数：3
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