Highly Efficient Capture and Electrochemical Release of Circulating Tumor Cells by Using Aptamers Modified Gold Nanowire Arrays

被引:72
作者
Zhai, Ting-Ting [1 ]
Ye, Dekai [1 ]
Zhang, Qian-Wen [1 ]
Wu, Zeng-Qiang [1 ]
Xia, Xing-Hua [1 ]
机构
[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
AuNWs; circulating tumor cells; capture; electrochemical release; topographical interaction; SELF-ASSEMBLED MONOLAYERS; SENSITIVE DETECTION; MULTIPLE TYPES; DESORPTION; SURFACES; PLATFORM; RECOGNITION; CATCH;
D O I
10.1021/acsami.7b11107
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
The effective capture and release of circulating tumor cells (CTCs) is of significant importance in cancer prognose and treatment. Here we report a highly efficient method to capture and release human leukemic lymphoblasts (CCRF-CEM) using aptamers modified gold nanowire arrays (AuNWs). The gold nanowires, showing tunable morphologies from relatively random pillar deposit to relatively uniform arrays, were fabricated by electrochemical deposition using anodic aluminum oxide (AAO) as template. Upon simply being modified with aptamers by Au-S chemistry, the AuNWs exhibit higher specificity to target cells. Also compared to flat gold substrate, the AuNWs with nanostructure can capture target cells with much higher capture yield. Moreover, the captured CCRF-CEM cells can be released from AuNWs efficiently with little damage through an electrochemical desorption process. We predict that our strategy has great potential in providing a simple and economical platform for CTCs isolation, cancer diagnosis, and therapy.
引用
收藏
页码:34706 / 34714
页数:9
相关论文
共 59 条
[1]   A vertically aligned carbon nanotube-based impedance sensing biosensor for rapid and high sensitive detection of cancer cells [J].
Abdolahad, Mohammad ;
Taghinejad, Mohammad ;
Taghinejad, Hossein ;
Janmaleki, Mohsen ;
Mohajerzadeh, Shams .
LAB ON A CHIP, 2012, 12 (06) :1183-1190
[2]   Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases [J].
Allard, WJ ;
Matera, J ;
Miller, MC ;
Repollet, M ;
Connelly, MC ;
Rao, C ;
Tibbe, AGJ ;
Uhr, JW ;
Terstappen, LWMM .
CLINICAL CANCER RESEARCH, 2004, 10 (20) :6897-6904
[3]   Synthesis of nano-textured biocompatible scaffolds from chicken eggshells [J].
Asghar, Waseem ;
Kim, Young-Tae ;
Ilyas, Azhar ;
Sankaran, Jeyantt ;
Wan, Yuan ;
Iqbal, Samir M. .
NANOTECHNOLOGY, 2012, 23 (47)
[4]   Electrical fingerprinting, 3D profiling and detection of tumor cells with solid-state micropores [J].
Asghar, Waseem ;
Wan, Yuan ;
Ilyas, Azhar ;
Bachoo, Robert ;
Kim, Young-tae ;
Iqbal, Samir M. .
LAB ON A CHIP, 2012, 12 (13) :2345-2352
[5]  
Bicelli LP, 2008, INT J ELECTROCHEM SC, V3, P356
[6]   Immune Readouts May Have Prognostic Value for the Course of Merkel Cell Carcinoma, a Virally Associated Disease [J].
Buck, Christopher B. ;
Lowy, Douglas R. .
JOURNAL OF CLINICAL ONCOLOGY, 2011, 29 (12) :1506-1508
[7]   Aptamer-Mediated Efficient Capture and Release of T Lymphocytes on Nanostructured Surfaces [J].
Chen, Li ;
Liu, Xueli ;
Su, Bin ;
Li, Jing ;
Jiang, Lei ;
Han, Dong ;
Wang, Shutao .
ADVANCED MATERIALS, 2011, 23 (38) :4376-+
[8]  
Cheng L, 2012, ACS NANO, V6, P5605, DOI [10.1021/nn301539m, 10.1021/nn304719q]
[9]   Applications of Aptamers as Sensors [J].
Cho, Eun Jeong ;
Lee, Joo-Woon ;
Ellington, Andrew D. .
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, 2009, 2 :241-264
[10]   Bioinspired Hierarchically Structured Surfaces for Efficient Capture and Release of Circulating Tumor Cells [J].
Dou, Xiaoqiu ;
Li, Ping ;
Jiang, Siyu ;
Bayat, Haider ;
Schoenherr, Holger .
ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (10) :8508-8518