The development of new biotechnologies using metalloprotein design

被引:43
作者
Benson, DE [1 ]
Wisz, MS [1 ]
Hellinga, HW [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
关键词
D O I
10.1016/S0958-1669(98)80010-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A variety of methodologies are under development to alter the behavior of existing metal centers or create entirely new sites within a protein framework in order to exploit the intrinsic chemical versatility of metals using the exquisite level of control that a protein matrix can exert to modulate their reactivity. Even at this relatively early stage, engineering of metal centers has led to the development of a number of emerging technologies with a wide variety of applications, including affinity purification of proteins, engineering of metal-mediated protein stability, control of protein activity, imaging and therapy, biosensors, and new catalysts.
引用
收藏
页码:370 / 376
页数:7
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