Role of EGF on in situ culture of equine preantral follicles and metabolomics profile

被引：20

作者：

Aguiar, F. L. N. ^{[1
]}

Lunardi, F. O. ^{[1
]}

Lima, L. F. ^{[1
]}

Bruno, J. B. ^{[1
]}

Alves, B. G. ^{[1
]}

Magalhaes-Padilha, D. M. ^{[2
]}

Cibin, F. W. S. ^{[3
]}

Berioni, L. ^{[4
]}

Apgar, G. A. ^{[5
]}

Lo Turco, E. G. ^{[4
]}

Gastal, E. L. ^{[5
]}

Figueiredo, J. R. ^{[1
]}

机构：

[1] Univ Estadual Ceara, Lab Manipulat Oocytes & Preantral Follicles LAMOF, Fortaleza, Ceara, Brazil

[2] Univ Potiguar, Laureate Int Univ, Postgrad Biotechnol, Av Senador Salgado Filho 1610, BR-59056000 Natal, RN, Brazil

[3] Fed Univ Pampa, Uruguaiana, RS, Brazil

[4] Sao Paulo Fed Univ, Dept Surg, Div Urol, Human Reprod Sect, Sao Paulo, SP, Brazil

[5] Southern Illinois Univ, Dept Anim Sci Food & Nutr, 1205 Lincoln Dr,MC 4417, Carbondale, IL 62901 USA

来源：

RESEARCH IN VETERINARY SCIENCE | 2017年 / 115卷

关键词：

In vitro culture; Equine preantral follicles; EGF; Metabolomics; Reactive oxygen species; EPIDERMAL-GROWTH-FACTOR; MALDI-TOF MS; VITRO CULTURE; STIMULATING-HORMONE; GRANULOSA-CELLS; ANTRUM FORMATION; FACTOR RECEPTOR; OVARIAN TISSUE; MESSENGER-RNA; LIPID PROFILE;

D O I：

10.1016/j.rvsc.2017.04.001

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The effects of epidermal growth factor (EGF) concentrations (0, 10, 50, and 100 ng/ml) on in vitro culture (IVC) of equine preantral follicles were evaluated using histology, estradiol and reactive oxygen species (ROS) production and metabolomics. After IVC, the percentage of normal follicles was lower (P < 0.05) for all treatments when compared to non-cultured control. EGF 50 ng/ml treatment had more (P < 0.05) normal follicles at Day 7 of culture when compared with EGF 0 and 100 ng/ml. EGF 50 ng/ml had more (P < 0.05) developing follicles than the 0 ng/ml and 10 ng/ml EGF treatments. Follicular and oocyte diameters were greater (P < 0.05) with EGF 50 ng/ml than the other cultured treatments, but similar (P > 0.05) to the non cultured control. From Day 1 to Day 7 estradiol production increased (P < 0.05) in all EGF treatments. EGF 50 ng/ml was the only treatment that maintained ROS production through IVC. Metabolomics profiles of the spent media indicated that eleven ions from variable influence in the projection (VIP) scores were higher represented in the EGF 50 ng/ml treatment. In conclusion, EGF 50 ng/ml treatment maintained follicle survival and ROS production, and promoted activation of cultured equine preantral follicles enclosed in ovarian tissue.

引用

页码：155 / 164

页数：10

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