Molecular characterization and biological activity of bovine interferon-omega3

被引：12

作者：

An, Dong ^{[1
]}

Guo, Yongli ^{[1
]}

Bao, Jun ^{[2
,3
]}

Luo, Xiuxin ^{[1
]}

Liu, Ying ^{[1
]}

Ma, Bo ^{[1
]}

Gao, Mingchun ^{[1
]}

Wang, Junwei ^{[1
,3
]}

机构：

[1] Northeast Agr Univ, Coll Vet Med, Dept Prevent Vet Med, Harbin 150030, Heilongjiang, Peoples R China

[2] Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Heilongjiang, Peoples R China

[3] Natl Food Safety & Nutr Collaborat Innovat Ctr, Wuxi 214122, Jiangsu, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2017年 / 115卷

关键词：

Bovine; IFN-omega; 3; Molecular characteristic; Antiviral activity; Interferon-stimulated genes; RECOMBINANT FELINE INTERFERON; YEAST PICHIA-PASTORIS; STIMULATED GENES; I INTERFERONS; PHYSICOCHEMICAL CHARACTERISTICS; ALPHA GENES; IFN-OMEGA; EXPRESSION; CLONING; THERAPY;

D O I：

10.1016/j.rvsc.2017.01.028

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine interferon-omega3 (BoIFN-omega 3) gene was amplified from bovine liver genomic DNA, which encodes a 195-amino acid protein containing a 23-amino acid signal peptide. Analysis of the molecular characteristics revealed that BoIFN-omega 3 evolving from IFN-omega, contained four cysteine residues and five alpha helices, showing that BolFN-omega 3 presented the typical molecular characteristics of type I interferon. BolFN-omega 3 exhibited antiviral and antiproliferative activities, which exerted a protective effect against VSV in several mammalian cell lines, as well as against BEV, IBRV, and BVDV in MDBK cell. Moreover, BolFN-omega 3 was shown to be highly sensitive to trypsin, but remaining stable despite changes in pH and temperature. Additionally, BoIFN-w3 induced the transcription of Mx1, ISG15, and ISG56 genes, as well as the expression of Mx1 protein in a time-dependent manner. These findings will be useful to further study BoIFN-omega in host's defence against infectious diseases, particularly viral infections. Furthermore, results will facilitate further research on the bovine interferon family. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：125 / 131

页数：7

共 45 条

[21] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].

Livak, KJ ;

Schmittgen, TD .

METHODS, 2001, 25 (04) :402-408

[22] Characterization and antivirus activities of a novel bovine IFN-omega24 [J].

Luo, Xiuxin ;

Guo, Yongli ;

Bao, Jun ;

Liu, Ying ;

An, Dong ;

Ma, Bo ;

Gao, Mingchun ;

Wang, Junwei .

MOLECULAR IMMUNOLOGY, 2015, 66 (02) :357-363

[23] Treatment of canine parvoviral enteritis with interferon-omega in a placebo-controlled challenge trial [J].

Martin, V ;

Najbar, W ;

Gueguen, S ;

Grousson, D ;

Eun, HM ;

Lebreux, B ;

Aubert, A .

VETERINARY MICROBIOLOGY, 2002, 89 (2-3) :115-127

[24]

Mege D., 1991, J INTERF RES, V11

[25] INHIBITORY EFFECTS OF RECOMBINANT FELINE INTERFERON ON THE REPLICATION OF FELINE ENTEROPATHOGENIC VIRUSES IN-VITRO [J].

MOCHIZUKI, M ;

NAKATANI, H ;

YOSHIDA, M .

VETERINARY MICROBIOLOGY, 1994, 39 (1-2) :145-152

[26] Sensitivity of FCV to recombinant feline interferon (rFeIFN) [J].

Ohe, Kyoko ;

Takahashi, Toshikazu ;

Hara, Daisuke ;

Hara, Motonobu .

VETERINARY RESEARCH COMMUNICATIONS, 2008, 32 (02) :167-174

[27] The evolution of the type I interferons [J].

Roberts, RM ;

Liu, LM ;

Guo, QT ;

Leaman, D ;

Bixby, J .

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1998, 18 (10) :805-816

[28] The bovine IFN-ω1 is biologically active and secreted at high levels in the yeast Pichia pastoris [J].

Rodríguez, M ;

Martínez, V ;

Alazo, K ;

Suárez, M ;

Redondo, M ;

Montero, C ;

Besada, V ;

de la Fuente, J .

JOURNAL OF BIOTECHNOLOGY, 1998, 60 (1-2) :3-14

[29] CONVENIENT ASSAY FOR INTERFERONS [J].

RUBINSTEIN, S ;

FAMILLETTI, PC ;

PESTKA, S .

JOURNAL OF VIROLOGY, 1981, 37 (02) :755-758

[30]

RYAN AM, 1993, ANIM GENET, V24, P9, DOI 10.1111/j.1365-2052.1993.tb00912.x

← 1 2 3 4 5 →