Molecular characterization and biological activity of bovine interferon-omega3

被引：12

作者：

An, Dong ^{[1
]}

Guo, Yongli ^{[1
]}

Bao, Jun ^{[2
,3
]}

Luo, Xiuxin ^{[1
]}

Liu, Ying ^{[1
]}

Ma, Bo ^{[1
]}

Gao, Mingchun ^{[1
]}

Wang, Junwei ^{[1
,3
]}

机构：

[1] Northeast Agr Univ, Coll Vet Med, Dept Prevent Vet Med, Harbin 150030, Heilongjiang, Peoples R China

[2] Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Heilongjiang, Peoples R China

[3] Natl Food Safety & Nutr Collaborat Innovat Ctr, Wuxi 214122, Jiangsu, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2017年 / 115卷

关键词：

Bovine; IFN-omega; 3; Molecular characteristic; Antiviral activity; Interferon-stimulated genes; RECOMBINANT FELINE INTERFERON; YEAST PICHIA-PASTORIS; STIMULATED GENES; I INTERFERONS; PHYSICOCHEMICAL CHARACTERISTICS; ALPHA GENES; IFN-OMEGA; EXPRESSION; CLONING; THERAPY;

D O I：

10.1016/j.rvsc.2017.01.028

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine interferon-omega3 (BoIFN-omega 3) gene was amplified from bovine liver genomic DNA, which encodes a 195-amino acid protein containing a 23-amino acid signal peptide. Analysis of the molecular characteristics revealed that BoIFN-omega 3 evolving from IFN-omega, contained four cysteine residues and five alpha helices, showing that BolFN-omega 3 presented the typical molecular characteristics of type I interferon. BolFN-omega 3 exhibited antiviral and antiproliferative activities, which exerted a protective effect against VSV in several mammalian cell lines, as well as against BEV, IBRV, and BVDV in MDBK cell. Moreover, BolFN-omega 3 was shown to be highly sensitive to trypsin, but remaining stable despite changes in pH and temperature. Additionally, BoIFN-w3 induced the transcription of Mx1, ISG15, and ISG56 genes, as well as the expression of Mx1 protein in a time-dependent manner. These findings will be useful to further study BoIFN-omega in host's defence against infectious diseases, particularly viral infections. Furthermore, results will facilitate further research on the bovine interferon family. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：125 / 131

页数：7

共 45 条

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