Studying Lipid Membrane Interactions of a Super-Cationic Peptide in Model Membranes and Living Bacteria

被引：3

作者：

Perez-Guillen, Isabel ^{[1
,2
]}

Domenech, Oscar ^{[2
,3
]}

Botet-Carreras, Adria ^{[2
,3
]}

Merlos, Alexandra ^{[1
]}

Sierra, Josep M. ^{[1
]}

Albericio, Fernando ^{[4
,5
]}

de la Torre, Beatriz G. ^{[6
]}

Montero, M. Teresa ^{[2
,3
]}

Vinas, Miguel ^{[1
]}

Borrell, Jordi H. ^{[2
,3
]}

机构：

[1] Univ Barcelona, Fac Med & Hlth Sci, Lab Mol Microbiol & Antimicrobials, Barcelona 08907, Spain

[2] Univ Barcelona, Fac Pharm & Food Sci, Phys Chem Sect, Barcelona 08028, Spain

[3] Univ Barcelona, Inst Nanosci & Nanotechnol IN2UB, Barcelona 08028, Spain

[4] Univ KwaZulu Natal, Sch Chem & Phys, Peptide Sci Lab, Univ Rd, ZA-4001 Durban, South Africa

[5] Univ Barcelona, Networking Ctr Bioengn Biomat & Nanomed, Dept Organ Chem, CIBER BBN, Barcelona 08028, Spain

[6] Univ KwaZulu Natal, Coll Hlth Sci, KRISP, ZA-4001 Durban, South Africa

来源：

PHARMACEUTICS | 2022年 / 14卷 / 10期

关键词：

super-cationic peptides; atomic force microscopy; anisotropy; antimicrobials interactions; ANTIMICROBIAL PEPTIDES; ANTIBIOTICS; DISCOVERY;

D O I：

10.3390/pharmaceutics14102191

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The super-cationic peptide dendrimers (SCPD) family is a valuable class of antimicrobial peptide candidates for the future development of antibacterial agents against multidrug-resistant gram-negative bacteria. The deep knowledge of their mechanism of action is a major challenge in research, since it may be the basis for future modifications/optimizations. In this work we have explored the interaction between SCPD and membranes through biophysical and microbiological approaches in the case of the G1OLO-L2OL2 peptide. Results support the idea that the peptide is not only adsorbed or close to the surface of the membrane but associated/absorbed to some extent to the hydrophobic-hydrophilic region of the phospholipids. The presence of low concentrations of the peptide at the surface level is concomitant with destabilization of the cell integrity and this may contribute to osmotic stress, although other mechanisms of action cannot be ruled out.

引用

页数：16

共 23 条

[1] When Combined with Colistin, an Otherwise Ineffective Rifampicin-Linezolid Combination Becomes Active in Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii [J].