The expression of SEIPIN in the mouse central nervous system

被引：9
作者：
Liu, Xiaoyun [1 ]
Xie, Beibei [2 ]
Qi, Yanfei [1 ]
Du, Ximing [1 ]
Wang, Shaoshi [3 ]
Zhang, Yumei [4 ]
Paxinos, George [5 ,6 ]
Yang, Hongyuan [1 ]
Liang, Huazheng [5 ,6 ]
机构：
[1] Univ New South Wales, Sch Biotechnol & Biomol Sci, Kensington, NSW, Australia
[2] Linyi Hosp, Dept Gynecol & Obstet, Linyi, Shangdong Provi, Peoples R China
[3] Shanghai 1 Peoples Hosp, Dept Neurol, Shanghai, Peoples R China
[4] Capital Med Univ, Beijing Tiantan Hosp, Dept Neurol, Beijing, Peoples R China
[5] Neurosci Res Australia, 139 Barker St, Randwick, NSW 2031, Australia
[6] Univ New South Wales, Sch Med Sci, Kensington, NSW, Australia
来源：
BRAIN STRUCTURE & FUNCTION | 2016年 / 221卷 / 08期
基金：
澳大利亚研究理事会; 英国医学研究理事会;
关键词：
SEIPIN; Brain; Supraspinal projection; Spinal cord; Seipinopathy; Motor control; CONGENITAL GENERALIZED LIPODYSTROPHY; ENDOPLASMIC-RETICULUM STRESS; SILVER-SYNDROME; MOTOR NEUROPATHY; MUTATION; BSCL2; GENE; PHENOTYPE; PROTEIN; NEURONS;
D O I：
10.1007/s00429-015-1151-3
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Immunohistochemical staining was used to investigate the expression pattern of SEIPIN in the mouse central nervous system. SEIPIN was found to be present in a large number of areas, including the motor and somatosensory cortex, the thalamic nuclei, the hypothalamic nuclei, the mesencephalic nuclei, some cranial motor nuclei, the reticular formation of the brainstem, and the vestibular complex. Double labeling with NeuN antibody confirmed that SEIPIN-positive cells in some nuclei were neurons. Retrograde tracer injections into the spinal cord revealed that SEIPIN-positive neurons in the motor and somatosensory cortex and other movement related nuclei project to the mouse spinal cord. The present study found more nuclei positive for SEIPIN than shown using in situ hybridization and confirmed the presence of SEIPIN in neurons projecting to the spinal cord. The results of this study help to explain the clinical manifestations of patients with Berardinelli-Seip congenital lipodystrophy (Bscl2) gene mutations.
引用
收藏
页码：4111 / 4127
页数：17
相关论文
共 27 条
  • [1] Phenotypic and genetic heterogeneity in congenital generalized lipodystrophy
    Agarwal, AK
    Simha, V
    Oral, EA
    Moran, SA
    Gorden, P
    O'Rahilly, S
    Zaidi, Z
    Gurakan, F
    Arslanian, SA
    Klar, A
    Ricker, A
    White, NH
    Bindl, L
    Herbst, K
    Kennel, K
    Patel, SB
    Al-Gazali, L
    Garg, A
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2003, 88 (10) : 4840 - 4847
  • [2] [Anonymous], 2013, MOUSE BRAIN STEREOTA
  • [3] The first Italian family with evidence of pyramidal impairment as phenotypic manifestation of Silver syndrome BSCL2 gene mutation
    Cafforio, Gianfranco
    Calabrese, Rosanna
    Morelli, Nicola
    Mancuso, Michelangelo
    Piazza, Selina
    Martinuzzi, Andrea
    Bassi, Maria Teresa
    Crippa, Francesco
    Siciliano, Gabriele
    [J]. NEUROLOGICAL SCIENCES, 2008, 29 (03) : 189 - 191
  • [4] Seipin ablation in mice results in severe generalized lipodystrophy
    Cui, Xin
    Wang, Yuhui
    Tang, Yin
    Liu, Yixiao
    Zhao, Liping
    Deng, Jingna
    Xu, Guoheng
    Peng, Xingui
    Ju, Shenghong
    Liu, George
    Yang, Hongyuan
    [J]. HUMAN MOLECULAR GENETICS, 2011, 20 (15) : 3022 - 3030
  • [5] Gene and phenotype analysis of congenital generalized lipodystrophy in Japanese: A novel homozygous nonsense mutation in seipin gene
    Ebihara, K
    Kusakabe, T
    Masuzaki, H
    Kobayashi, N
    Tanaka, T
    Chusho, H
    Miyanaga, F
    Miyazawa, T
    Hayashi, T
    Hosoda, K
    Ogawa, Y
    Nakao, K
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2004, 89 (05) : 2360 - 2364
  • [6] Seipin, adipogenesis and lipid droplets
    Fei, Weihua
    Du, Ximing
    Yang, Hongyuan
    [J]. TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2011, 22 (06) : 204 - 210
  • [7] Neuroanatomical Characterisation of the Expression of the Lipodystrophy and Motor-Neuropathy Gene Bscl2 in Adult Mouse Brain
    Garfield, Alastair S.
    Chan, Wai S.
    Dennis, Rowena J.
    Ito, Daisuke
    Heisler, Lora K.
    Rochford, Justin J.
    [J]. PLOS ONE, 2012, 7 (09):
  • [8] A new seipin-associated neurodegenerative syndrome
    Guillen-Navarro, Encarna
    Sanchez-Iglesias, Sofia
    Domingo-Jimenez, Rosario
    Victoria, Berta
    Ruiz-Riquelme, Alejandro
    Rabano, Alberto
    Loidi, Lourdes
    Beiras, Andres
    Gonzalez-Mendez, Blanca
    Ramos, Adriana
    Lopez-Gonzalez, Vanesa
    Juliana Ballesta-Martinez, Maria
    Garrido-Pumar, Miguel
    Aguiar, Pablo
    Ruibal, Alvaro
    Requena, Jesus R.
    Araujo-Vilar, David
    [J]. JOURNAL OF MEDICAL GENETICS, 2013, 50 (06) : 401 - 409
  • [9] Motor neuron degeneration in a mouse model of seipinopathy
    Guo, J.
    Qiu, W.
    Soh, S. L. Y.
    Wei, S.
    Radda, G. K.
    Ong, W-Y
    Pang, Z. P.
    Han, W.
    [J]. CELL DEATH & DISEASE, 2013, 4 : e535 - e535
  • [10] The phenotype of motor neuropathies associated with BSCL2 mutations is broader than Silver syndrome and distal HMN type V
    Irobi, J
    Van den Bergh, P
    Merlini, L
    Verellen, C
    Van Maldergem, L
    Dierick, I
    Verpoorten, N
    Jordanova, A
    Windpassinger, C
    De Vriendt, E
    Van Gerwen, V
    Auer-Grumbach, M
    Wagner, K
    Timmerman, V
    De Jonghe, P
    [J]. BRAIN, 2004, 127 : 2124 - 2130
123