Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies

被引：10

作者：

Dai, Jun ^{[1
]}

Zhang, Cai ^{[1
]}

Tian, Zhigang ^{[1
]}

Zhang, Jian ^{[1
]}

机构：

[1] Shandong Univ, Inst Immunopharmacol & Immunotherapy, Sch Pharmaceut Sci, Jinan 250012, Shandong, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2011年 / 2卷 / 03期

基金：

中国国家自然科学基金;

关键词：

homeobox containing 1; monoclonal antibody; immunoanalytical methods; carcinoma; PROTEIN; ACTIVATION;

D O I：

10.3892/etm.2011.240

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Homeobox containing 1 (HMBOX1) is a novel transcription factor. However, the expression of HMBOX1 and its functions in human cancer tissues and cell lines have not been fully defined. We generated two specific monoclonal antibodies, 2A5F4 and 4A4F2, against human HMBOX1. In the present study, these two anti-HMBOX1 antibodies were used to investigate the protein expression profile of HMBOX I in various human cancer tissues and cell lines. The results showed that HMBOX1 in kidney tissue was mainly expressed in the renal tubule; the expression level of HMBOX1 was much higher in clear-cell carcinoma of the kidney originating from the renal tubule. Additionally, high levels of HMBOXI protein were detected not only in pancreatic cancer tissue but also in the adjacent normal tissue. Notably, the expression level of HMBOX1 in liver cancer was dramatically decreased compared with that in the adjacent normal tissue. Abnormal expression of HMBOX1 in different types of carcinoma tissues suggests that HMBOX1 may be involved in the pathobiology of tumors.

引用

页码：487 / 490

页数：4

共 13 条

[1] Hepatic nuclear factor 1-α: inflammation, genetics, and atherosclerosis [J].

Armendariz, Angela D. ;

Krauss, Ronald M. .

CURRENT OPINION IN LIPIDOLOGY, 2009, 20 (02) :106-111

[2] ISOLATION OF HUMAN AND CANINE IGM UTILIZING PROTEIN A AFFINITY-CHROMATOGRAPHY [J].

BALINT, JP ;

IKEDA, Y ;

NAGAI, T ;

TERMAN, DS .

IMMUNOLOGICAL COMMUNICATIONS, 1981, 10 (06) :533-540

[3] Isolation and functional analysis of human HMBOX1, a homeobox containing protein with transcriptional repressor activity [J].

Chen, S. ;

Saiyin, H. ;

Zeng, X. ;

Xi, J. ;

Liu, X. ;

Li, X. ;

Yu, L. .

CYTOGENETIC AND GENOME RESEARCH, 2006, 114 (02) :131-136

[4] Recombinant Expression of a Novel Human Transcriptional Repressor HMBOX1 and Preparation of Anti-HMBOX1 Monoclonal Antibody [J].

Dai, Jun ;

Wu, Longyan ;

Zhang, Cai ;

Zheng, Xiaodong ;

Tian, Zhigang ;

Zhang, Jian .

CELLULAR & MOLECULAR IMMUNOLOGY, 2009, 6 (04) :261-268

[5] Transcriptional coactivators CBP and p300 cooperatively enhance HNF-1α-mediated expression of the albumin gene in hepatocytes [J].

Dohda, T ;

Kaneoka, H ;

Inayoshi, Y ;

Kamihira, M ;

Miyake, K ;

Iijima, S .

JOURNAL OF BIOCHEMISTRY, 2004, 136 (03) :313-319

[6] HOMEOBOX GENES - THEIR FUNCTION IN DROSOPHILA SEGMENTATION AND PATTERN-FORMATION [J].

LAWRENCE, PA ;

MORATA, G .

CELL, 1994, 78 (02) :181-189

[7] Maturity-onset diabetes of the young: from clinical description to molecular genetic characterization [J].

Owen, K ;

Hattersley, AT .

BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM, 2001, 15 (03) :309-323

[8] Neurogenin3 and hepatic nuclear factor 1 cooperate in activating pancreatic expression of Pax4 [J].

Smith, SB ;

Gasa, R ;

Watada, H ;

Wang, JH ;

Griffen, SC ;

German, MS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (40) :38254-38259

[9] The bifunctional DCOH protein binds to HNF1 independently of its 4-alpha-carbinolamine dehydratase activity [J].

Sourdive, DJD ;

Transy, C ;

Garbay, S ;

Yaniv, M .

NUCLEIC ACIDS RESEARCH, 1997, 25 (08) :1476-1484

[10] Transcriptional activation by hepatocyte nuclear factor-1 requires synergism between multiple coactivator proteins [J].

Soutoglou, E ;

Papafotiou, G ;

Katrakili, N ;

Talianidis, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (17) :12515-12520

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