The Novel PRRSV Strain HBap4-2018 with a Unique Recombinant Pattern Is Highly Pathogenic to Piglets

被引：30

作者：

Chen, Pengfei ^{[1
,3
]}

Tan, Xiangmei ^{[1
]}

Lao, Mengqin ^{[1
]}

Wu, Xia ^{[1
]}

Zhao, Xiongwei ^{[1
]}

Zhou, Shuting ^{[1
,2
]}

Yu, Jiarong ^{[1
]}

Zhu, Junrui ^{[1
]}

Yu, Lingxue ^{[1
]}

Tong, Wu ^{[1
]}

Gao, Fei ^{[1
]}

Yu, Hai ^{[1
]}

Liu, Changlong ^{[1
]}

Jiang, Yifeng ^{[1
]}

Tong, Guangzhi ^{[1
,3
]}

Zhou, Yanjun ^{[1
,3
]}

机构：

[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Dept Swine Infect Dis, Shanghai 200241, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai Key Lab Vet Biotechnol, Shanghai 200240, Peoples R China

[3] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

来源：

VIROLOGICA SINICA | 2021年 / 36卷 / 06期

基金：

中国国家自然科学基金; 上海市自然科学基金;

关键词：

Porcine reproductive and respiratory syndrome virus (PRRSV); Genetic variation; Nsp2; Recombination; Pathogenicity; RESPIRATORY SYNDROME VIRUS; EFFICACY EVALUATION; GENETIC-CHARACTERIZATION; NADC30-LIKE STRAIN; VACCINES; EMERGENCE; CHINA; IDENTIFICATION; REGION; NSP2;

D O I：

10.1007/s12250-021-00453-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Currently, various porcine reproductive and respiratory syndrome virus (PRRSV) variants emerged worldwide with different genetic characteristics and pathogenicity, increasing the difficulty of PRRS control. In this study, a PRRSV strain named HBap4-2018 was isolated from swine herds suffering severe respiratory disease with high morbidity in Hebei Province of China in 2018. The genome of HBap4-2018 is 15,003 nucleotides in length, and compared with NADC30-like PRRSV, nsp2 of HBap4-2018 has an additional continuous deletion of five amino acids. Phylogenetic analysis based on complete genome and ORF5 showed that HBap4-2018 belonged to lineage 8 of PRRSV-2, which was characterized by highly variable genome. However, HBap4-2018 was classified into lineage 1 based on phylogenetic analysis of nsp2, sharing higher amino acid homology (85.3%-85.5%) with NADC30-like PRRSV. Further analysis suggested that HBap4-2018 was a novel natural recombinant PRRSV with three recombinant fragments in the genome, of which highly pathogenic PRRSV (HP-PRRSV) served as the major parental strains, while NADC30-like PRRSV served as the minor parental strains. Five recombination break points were identified in nsp2, nsp3, nsp5, nsp9 and ORF6, respectively, presenting a novel recombinant pattern in the genome. Piglets inoculated with HBap4-2018 presented typical clinical signs with a mortality rate of 60%. High levels of viremia and obvious macroscopic and histopathological lesions in the lungs were observed, revealing the high pathogenicity of HBap4-2018 in piglets.

引用

页码：1611 / 1625

页数：15

共 62 条

[51] Characterization of newly emerged NADC30-like strains of porcine reproductive and respiratory syndrome virus in China [J].