An enzymatic flow analysis method for the determination of phosphatidylcholine in sediment pore waters and extracts

被引：18

作者：

Amini, N ^{[1
]}

McKelvie, I ^{[1
]}

机构：

[1] Monash Univ, Water Studies Ctr, Sch Chem, Clayton, Vic 3800, Australia

来源：

TALANTA | 2005年 / 66卷 / 02期

关键词：

phospholipase C; alkaline phosphatase; choline oxidase; immobilized enzyme(s); luminol;

D O I：

10.1016/j.talanta.2004.11.001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and selective flow injection method for the determination of phosphatdylcholine (PC) in sediment pore waters and extracts is described. It involves the use of phospholipase C, alkaline phosphatase and choline oxidase co-immobilized on controlled pore glass in a packed column reactor. The final product of the enzymatic reaction of phosphatidylcholine is hydrogen peroxide, and this is detected by measuring the chemiluminescence emission resulting from cobalt(II) catalysed reaction with luminol. The flow injection method is rapid (30 injections/h), reproducible (1.4% R.S.D. at 3 mu M PC, n=10) with a detection limit of 0.14 mu M (estimated from 3 sigma(n-1) of the measured blank). A linear calibration response was obtained over a concentration range of 0.5-9 mu M (r=0.999). The method has been applied to the determination of phosphatidylcholine in sediment extracts and sediment pore waters. (c) 2004 Elsevier B.v. All rights reserved.

引用

页码：445 / 452

页数：8