Hepatic CCR7lowCD62lowCD45RClow allograft dendritic cells migrate to the splenic red pulp in immunologically unresponsive rats

被引:6
作者
Furuhashi, T
Yamaguchi, Y
Wang, FS
Uchino, S
Okabe, K
Ohshiro, H
Kihara, S
Yamada, S
Mori, K
Ogawa, M
机构
[1] Kumamoto Univ, Sch Med, Dept Surg 2, Kumamoto 8608556, Japan
[2] Kumamoto Univ, Sch Med, Dept Educ, Kumamoto 8608556, Japan
[3] Univ Occupat & Environm Hlth, Dept Internal Med, Kitakyushu, Fukuoka 807, Japan
关键词
dendritic cells; chemokine receptors; leukocyte common antigen; CD45; blood transfusion; immunologic unresponsiveness; liver transplantation;
D O I
10.1016/j.jss.2004.08.030
中图分类号
R61 [外科手术学];
学科分类号
摘要
Donor dendritic cells (DC) migrate into the recipient spleen after hepatic transplantation. Immunological unresponsiveness to rat hepatic allografts can be induced by prior donor-specific blood transfusion (DST). We investigated homing receptor phenotype and splenic distribution of donor DC after allografting and DST. Immunostaining revealed OX62(+) cells in the splenic red pulp of animals receiving pre-transplant DST but only in the white pulp of untreated animals. Most OX62 cells were positive for OX76. There were two subsets of DC in the spleen, CD45RC(high)OX62(+) and CD45RC(low)OX62(+) cells. RT-PCR revealed that CD45RC(low)OX62(+) cells expressed interleukin (IL)-10, while CD45RC(high)OX62(+) cells expressed IL-2 and low levels of IL-10 mRNA. CD45RC(high)OX62(+) cells strongly expressed CCR5 and CCR7, compared with weak expression in CD45RC(low)OX62(+) cells. The Epstein-Barr virus-induced molecule 1 (EBI-1) ligand chemokine (ELC/MIP3 beta) was expressed mainly within the splenic white pulp. Mucosal vascular addressin-cell adhesion molecule-1 (MAdCAM-1) was expressed in the marginal zone and white pulp, but expression of splenic M.AdCAM-1 was down-regulated in DST-treated animals. L-selectin (CD62L), the ligand for MAdCAM-1, was strongly expressed on CD45RC(high)OX62(+) cells but not on CD45RC(low)OX62(+) cells. In conclusion, differential splenic. migration of CCR5(low)CCR7(low)CD62L(low)CD45RC(low) DC expressing Th2-type cytokines is associated with immunological unresponsiveness to rat hepatic allografts. (c) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:29 / 37
页数:9
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