Mass Analyzers and Mass Spectrometers

被引：41

作者：

Haag, Anthony M. ^{[1
,2
]}

机构：

[1] Baylor Coll Med, Dept Pathol & Immunol, Houston, TX 77030 USA

[2] Texas Childrens Hosp, Texas Childrens Microbiome Ctr, Houston, TX 77030 USA

来源：

MODERN PROTEOMICS - SAMPLE PREPARATION, ANALYSIS AND PRACTICAL APPLICATIONS | 2016年 / 919卷

关键词：

Mass spectrometer; Mass analyzer; Quadrupole mass analyzer; Ion trap mass analyzer; Time-of-flight (TOF) mass analyzer; FT-ICR mass analyzer; Orbitrap mass analyzer; Tandem mass analyzer; Triple quadrupoles tandem mass analyzer; Q-TOF tandem mass analyzer; TOF/TOF tandem mass analyzer; Product ion scan; Precursor ion scan; Neutral loss scan; Selected reaction monitoring; LASER-DESORPTION IONIZATION; COLLISION-INDUCED DISSOCIATION; ELECTROSPRAY-IONIZATION; LARGE BIOMOLECULES; QUADRUPOLE; PROTEINS; IDENTIFICATION; COMPLEXES; ORBITRAP; PRIMER;

D O I：

10.1007/978-3-319-41448-5_7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometers are comprised of three main components: an ion source, a mass analyzer, and a detector. Ionization of the analyte occurs in the ion source and the resulting ions are counted at the detector. However, it is the mass analyzer that is responsible for determing the mass-to-charge ratio (m/z) of the ions (Jennings KR, Dolnikowski GG, Method Enzymol 193:37-61, 1990). Therefore, it is primarily the analyzer that allows the mass spectrometer to serve its primary goal-determining the mass of the analytes being measured. This becomes important in the field of molecular biology, where biomolecules may be of low molecular weight or often take on multiple charges (z) after ionization (Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM, Science 246:64-71, 1989). For this reason, the choice of analyzer is dependant on the properties of the analyte after ionization and the requirements of the experiment being performed.

引用

页码：157 / 169

页数：13