Intrinsic viscosity of bovine serum albumin conformers

被引：49

作者：

Curvale, Rolando ^{[1
,2
]}

Masuelli, Martin ^{[1
]}

Padilla, Antonio Perez ^{[2
]}

机构：

[1] Univ Nacl San Luis, Fac Quim Bioquim & Farmacia, Area Fis Quim, San Luis, Argentina

[2] Consejo Nacl Invest Cient & Tecn, Inst Invest Tecnol Quim, RA-5700 San Luis, Argentina

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2008年 / 42卷 / 02期

关键词：

bovine serum albumin (BSA); intrinsic viscosity; conformers; Huggins' constant; Kraemer's constant; Mark-Houwink's constant;

D O I：

10.1016/j.ijbiomac.2007.10.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intrinsic viscosity of bovine serum albumin (BSA) at different pH values (2.7, 4.3, 7.4, 8 and 10) has been determined, as well as the Mark-Houwink constant and expansion factor. The traditional technique for data analysis using extrapolation to obtain intrinsic viscosity values shows an unusual behavior regarding concentration that can be observed in the values obtained for Huggins' and Kraemer's constants. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：133 / 137

页数：5

共 29 条

[1] STRUCTURE OF BOVINE SERUM ALBUMIN AT LOW PH [J].

BLOOMFIELD, V .

BIOCHEMISTRY, 1966, 5 (02) :684-+

[2]

CARTER DC, 1994, ADV PROTEIN CHEM, V45, P153

[3]

CILUUZO F, 2005, AAPS PHARMSCITECH, pE586

[4] SOLPRO: Theory and computer program for the prediction of SOLution PROperties of rigid macromolecules and bioparticles [J].

delaTorre, JG ;

Carrasco, B ;

Harding, SE .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1997, 25 (5-6) :361-372

[5] Viscometric study of poly(methyl methacrylate-g-propylene oxide) and respective homopolymers [J].

Delpech, MC ;

Oliveira, CMF .

POLYMER TESTING, 2005, 24 (03) :381-386

[6] The conformation of serum albumin in solution:: A combined phosphorescence depolarization-hydrodynamic modeling study [J].

Ferrer, ML ;

Duchowicz, R ;

Carrasco, B ;

de la Torre, JG ;

Acuña, AU .

BIOPHYSICAL JOURNAL, 2001, 80 (05) :2422-2430

[7]

Flecha FLG, 2003, BIOCHEM MOL BIOL EDU, V31, P319

[8]

Foster JF, 1977, Albumin: Structure, Function and Uses, P53, DOI DOI 10.1016/B978-0-08-019603-9.50010-7

[9] PHYSICAL AND BINDING PROPERTIES OF LARGE FRAGMENTS OF HUMAN-SERUM ALBUMIN [J].

GEISOW, MJ ;

BEAVEN, GH .

BIOCHEMICAL JOURNAL, 1977, 163 (03) :477-484

[10] ANOMALOUS VISCOSITY BEHAVIOUR OF BLOCK COPOLYMERS AS A FUNCTION OF TEMPERATURE FOR 2 SEQUENCE OR AB TYPE POLY(ISOPRENE-STYRENE) [J].

GIROLAMO, M ;

URWIN, JR .

EUROPEAN POLYMER JOURNAL, 1971, 7 (06) :693-&

← 1 2 3 →