Subgingival microbiota in health compared to periodon tis and the influence of smoking

被引:153
作者
Camelo-Castillo, Anny J. [1 ]
Mira, Alex [1 ]
Pico, Alex [2 ]
Nibali, Luigi [3 ]
Henderson, Brian [4 ]
Donos, Nikolaos [3 ]
Tomas, Inmaculada [2 ]
机构
[1] Fomento Invest Sanitaria & Biomed Comunitat Valan, Ctr Adv Res Publ Hlth, Dept Hlth & Genom, Valencia, Spain
[2] Univ Santiago Compostela, Sch Med & Dent, Oral Sci Res Grp, Special Needs Unit, E-15706 Santiago, Spain
[3] UCL, Eastman Dent Inst, Dept Clin Res, Periodontol Unit, London, England
[4] UCL, Eastman Dent Inst, Dept Microbial Dis, London, England
来源
FRONTIERS IN MICROBIOLOGY | 2015年 / 6卷
关键词
16S ribosomal RNA; chronic periodontitis; dental plague; microbiome; oral health; smoking; AGGRESSIVE PERIODONTITIS; FILIFACTOR-ALOCIS; ORAL MICROBIOTA; DENTAL-CARIES; DISEASE; GENERATION; DIVERSITY; PROFILES; PLAQUE; IDENTIFICATION;
D O I
10.3389/fmicb.2015.00119
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The etiology of periodontitis has traditionally been associated to a consortium of three bacterial species the so-called "red-complex" of periodontal disease which has been the target for most diagnostic and therapeutic strategies. However, other species have also been found to correlate with disease severity. In addition, the influence of smoking on periodontal microbiota is poorly understood. In the current manuscript, the composition of the subgingival microbiota in healthy individuals vs. patients with chronic periodontitis has been investigated using 16S pyrosequencing and the influence of smoking on periodontal composition has been examined. Subgingival bacterial communities were sampled from 82 patients: 22 non-smoking healthy controls, 28 non-smoking periodontal patients, and 32 smoking periodontal patients. Bacterial diversity was higher in periodontal patients than in healthy subjects, which could be interpreted as the consequence of a nutritionally richer environment or a reduced immune competence. Periodontal patients showed a significantly higher prevalence/relative abundance of "established" periopathogens but also other taxa whose role is not well-established and that should be targets for future research. These include Anaeroglobus, Bulleidia, Desulfobulbus, Filifactor, Mogibacterium, Phocaeicola, Schwartzia or TM7 The microbial community of smoking-associated periodontitis is less diverse and distinct from that of non-smokers, indicating that smoking has an influence on periodontal ecology. Interestingly, the high sequencing coverage allowed the detection at low proportions of periodontal pathogens in all healthy individuals, indicating that chronic periodontitis cannot be strictly considered an infectious disease but the outcome of a polymicrobial dysbiosis, where changes in the proportions of microbial consortia trigger the inflammatory and tissue-degradation responses of the host.
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页数:12
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