The effect of feeding dehulled cottonseed protected from ruminal metabolism (PCS): together with supplements of protected sunflower seed meal (PSM), on the performance and fatty acid profile of adipose tissue in feedlot steers was examined. Sixty-two Hereford cross steers, average weight 470 kg, were assigned to 4 groups and fed rations based on barley-corn silage for 150 days. The diets offered contained 10% untreated whole cottonseed + 6% untreated sunflower seed meal (Group I), 10% PCS + 10% PSM (Group II): 15% PCS + 10% PSM (Group III), or 20% PCS + 10% PSM (Group IV). Inclusion of PCS at the level of 10, 15, and 20% together with 10% PSM resulted in an increase (P < 0.01) in dressing percentage together with an improvement in feed conversion ratio. There was a 5-8-fold increase in the proportion of linoleic acid (C18:2) in omental, perirenal, and subcutaneous fat from cattle fed the PCS supplement. A significant reduction occurred in the proportion of oleic acid (C18:1 cis), but the percentage of palmitic acid (C16:0) was not affected; the proportion of stearic acid (C18:0) in subcutaneous fat increased with PCS supplementation.