Electrochemical properties of myoglobin deposited on multi-walled carbon nanotube/ciprofloxacin film

被引：12

作者：

Kumar, S. Ashok ^{[1
]}

Wang, Sea-Fue ^{[1
]}

Chang, Yu-Tsern ^{[2
]}

Lu, His-Chuan ^{[1
]}

Yeh, Chun-Ting ^{[1
]}

机构：

[1] Natl Taipei Univ Technol, Dept Mat & Mineral Resources Engn, Taipei, Taiwan

[2] Nanya Inst Technol, Dept Chem & Mat Engn, Jhongli 32091, Taiwan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2011年 / 82卷 / 02期

关键词：

Myoglobin; CNTs; Surfaces; Thin films; Nanomaterials; Direct electrochemistry; BY-LAYER FILMS; DIRECT ELECTRON-TRANSFER; IONIC LIQUID ELECTRODE; NANOTUBES MODIFIED ELECTRODE; HEME-PROTEINS; GOLD NANOPARTICLES; ELECTROCATALYSIS; SURFACTANTS; BIOSENSOR; NANOHYBRID;

D O I：

10.1016/j.colsurfb.2010.10.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report the direct electrochemical and electrocatalytic properties of myoglobin (MB) on a multi-walled carbon nanotube/ciprofloxacin (MWCNT/CF) film-modified electrode. A highly homogeneous MWCNT thin-film was prepared on an electrode surface using ciprofloxacin (CF) as a dispersing agent. MB was then electrochemically deposited onto the MWCNT/CF-modified electrode. The MB/MWCNT/CF film was characterized by scanning electron microscopy and UV-visible spectroscopy (UV-vis). UV-vis spectra confirmed that MB retained its original state on the MWCNT/CF film. Direct electrochemical properties of MB on the MWCNT/CF film were investigated by cyclic voltammetry. The formal potential and electron transfer rate constant were evaluated in pH 7.2 buffer solution as -0.327 V and 300 s(-1), respectively. In addition, the MB/MWCNT/CF-modified electrode showed excellent electrocatalytic properties for the reduction of hydrogen peroxide (H2O2). The MB/MWCNT/CF-modified electrode was used for the detection of H2O2 at concentrations from 1 x 10(-6) M to 7 x 10(-4) M in pH 7.2 buffer solution. Overall, the MB/MWCNT/CF-modified electrode was very stable and has potential for development as a H2O2 sensor. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：526 / 531

页数：6

共 43 条

[1] Electrochemical Sensors Based on Carbon Nanotubes [J].

Ahammad, A. J. Saleh ;

Lee, Jae-Joon ;

Rahman, Md. Aminur .

SENSORS, 2009, 9 (04) :2289-2319

[2] Catalytic activity of myoglobin immobilized on zirconium phosphonates [J].

Bellezza, F ;

Cipiciani, A ;

Costantino, U ;

Nicolis, S .

LANGMUIR, 2004, 20 (12) :5019-5025

[3] Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection [J].

Cai, H ;

Cao, XN ;

Jiang, Y ;

He, PG ;

Fang, YZ .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 375 (02) :287-293

[4] Direct electrochemistry and electrocatalysis of myoglobin immobilized on gold nanoparticles/carbon nanotubes nanohybrid film [J].

Cao, Wei ;

Wei, Cuimei ;

Hu, Jingbo ;

Li, Qilong .

ELECTROANALYSIS, 2008, 20 (17) :1925-1931

[5] Direct electrochemistry of heme proteins: effect of electrode surface modification by neutral surfactants [J].

Chattopadhyay, K ;

Mazumdar, S .

BIOELECTROCHEMISTRY, 2001, 53 (01) :17-24

[6] Direct electrochemical response of myoglobin using a room temperature ionic liquid, 1-(2-hydroxyethyl)-3-methyl imidazolium tetrafluoroborate, as supporting electrolyte [J].

Ding, Su-Fang ;

Xu, Miao-Qing ;

Zhao, Guang-Chao ;

Wei, Xian-Wen .

ELECTROCHEMISTRY COMMUNICATIONS, 2007, 9 (02) :216-220

[7] Preparation and characterization of room temperature ionic liquid/single-walled carbon nanotube nanocomposites and their application to the direct electrochemistry of heme-containing proteins/enzymes [J].

Du, Pan ;

Liu, Shuna ;

Wu, Ping ;

Cai, Chenxin .

ELECTROCHIMICA ACTA, 2007, 52 (23) :6534-6547

[8] Recognition of Different Environments at the Porous Cage of CTAB Based Ormosils [J].

Maria L. Ferrer ;

Francisco del Monte ;

David Levy .

Journal of Sol-Gel Science and Technology, 2003, 26 (1-3) :353-356

[9] Direct electrochemistry of myoglobin based on DNA accumulation on carbon ionic liquid electrode [J].

Gao, Ruifang ;

Zheng, Jianbin .

ELECTROCHEMISTRY COMMUNICATIONS, 2009, 11 (07) :1527-1529

[10] ELECTRICAL WIRING OF REDOX ENZYMES [J].

HELLER, A .

ACCOUNTS OF CHEMICAL RESEARCH, 1990, 23 (05) :128-134

← 1 2 3 4 5 →