Synthesis, characterization and antitumoral activity of new cobalt(II)complexes: Effect of the ligand isomerism on the biological activity of the complexes

The synthesis, physico-chemical characterization and cytotoxicity against five human tumoral cell lines (THP-1, U937, Molt-4, Colo205 and H460) of three new cobalt(II) coordination compounds are reported (i.e. Co(HL1)Cl (1), Co(HL2)Cl (2) and [Co(HL3)Cl]0.0.5 (CH3)(2)CHOH (3)). H(2)L2 (2-([[2-hydroxy-3-(1-naphthyloxy)propyl](pyridin-2-ylmethyl)amino]methyl}phenol) and H(2)L3 (2-{[[2-hydroxy-3-(2naphthyloxy)propyl](pyridin-2-ylmethyl)amino]methyl}phenol) present alpha and beta-naphthyl groups respectively, which is absent in H(2)L1 (N-(2-hydroxybenzyl)-N-(2-pyridylmethyl)[(3-chloro)(2-hydroxy)]propylamine. These compounds were characterized by a range of physico-chemical methods. X-ray diffraction studies were performed for complex (3), indicating the formation of a mononuclear complex. Complexes (2) and (3), which contain alpha and beta-naphthyl groups respectively, have presented lower IC50 values than those exhibited by complex (1). Complex (3) presents IC50 values lower than cisplatin against Colo205 (90 and 196 mu mol L-1, respectively) and H460 (147 and 197 mu mol L-1, respectively). These human neoplastic cells under investigation were also more susceptible toward complex (3) than peripheral blood mononuclear cells. Transmission electron microscopy investigations are in agreement with the loss of mitochondrial membrane potential (Delta Psi m) observed by JC-1 mitochondrial potential sensor and indicate that the activity of complex (3) against leukemic cell line (13937) is mediated by an apoptotic mechanism associated with mitochondrial dysfunction (intrinsic pathway). (C) 2016 Elsevier Inc. All rights reserved.