Cancer and genomics

被引：100

作者：

Futreal, PA

Kasprzyk, A

Birney, E

Mullikin, JC

Wooster, R

Stratton, MR

机构：

[1] Sanger Ctr, Canc Genome Project, Cambridge CB10 1SA, England

[2] Sanger Ctr, Informat Div, Cambridge CB10 1SA, England

[3] EBI, EMBL, Cambridge CB10 1SD, England

[4] Inst Canc Res, Sutton SM2 5NG, Surrey, England

来源：

NATURE | 2001年 / 409卷 / 6822期

关键词：

D O I：

10.1038/35057046

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Identification of the genes that cause oncogenesis is a central aim of cancer research. We searched the proteins predicted from the draft human genome sequence for paralogues of known tumour suppressor genes, but no novel genes were identified. We then assessed whether it was possible to search directly for oncogenic sequence changes in cancer cells by comparing cancer genome sequences against the draft genome. Apparently chimaeric transcripts (from oncogenic fusion genes generated by chromosomal translocations, the ends of which mapped to different genomic locations) were detected to the same degree in both normal and neoplastic tissues, indicating a significant level of false positives. Our experiment underscores the limited amount and variable quality of DNA sequence from cancer cells that is currently available.

引用

页码：850 / 852

页数：3

共 6 条

[1]

Bell RS, 1999, CAN J SURG, V42, P259

[2] Using GeneWise in the Drosophila annotation experiment [J].

Birney, E ;

Durbin, R .

GENOME RESEARCH, 2000, 10 (04) :547-548

[3]

Higginson J., 1992, Human cancer: epidemiology and environmental causes

[4] The p53 gene family [J].

Kaelin, WG .

ONCOGENE, 1999, 18 (53) :7701-7705

[5] Initial sequencing and analysis of the human genome [J].

Lander, ES ;

Int Human Genome Sequencing Consortium ;

Linton, LM ;

Birren, B ;

Nusbaum, C ;

Zody, MC ;

Baldwin, J ;

Devon, K ;

Dewar, K ;

Doyle, M ;

FitzHugh, W ;

Funke, R ;

Gage, D ;

Harris, K ;

Heaford, A ;

Howland, J ;

Kann, L ;

Lehoczky, J ;

LeVine, R ;

McEwan, P ;

McKernan, K ;

Meldrim, J ;

Mesirov, JP ;

Miranda, C ;

Morris, W ;

Naylor, J ;

Raymond, C ;

Rosetti, M ;

Santos, R ;

Sheridan, A ;

Sougnez, C ;

Stange-Thomann, N ;

Stojanovic, N ;

Subramanian, A ;

Wyman, D ;

Rogers, J ;

Sulston, J ;

Ainscough, R ;

Beck, S ;

Bentley, D ;

Burton, J ;

Clee, C ;

Carter, N ;

Coulson, A ;

Deadman, R ;

Deloukas, P ;

Dunham, A ;

Dunham, I ;

Durbin, R ;

French, L .

NATURE, 2001, 409 (6822) :860-921

[6] The critical role of chromosome translocations in human leukemias [J].

Rowley, JD .

ANNUAL REVIEW OF GENETICS, 1998, 32 :495-+

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