Cell type-specific chromatin structure determines the targeting of V(D)J recombinase activity in vitro

被引：256
|
作者：
StanhopeBaker, P [1 ]
Hudson, KM [1 ]
Shaffer, AL [1 ]
Constantinescu, A [1 ]
Schlissel, MS [1 ]
机构：
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205
来源：
CELL | 1996年 / 85卷 / 06期
关键词：
D O I：
10.1016/S0092-8674(00)81272-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A common V(D)J recombinase that recognizes a conserved recombination signal sequence (RSS) mediates the assembly of immunoglobulin (Ig) and T cell receptor (TCR) genes in B and T cell precursors. The rearrangement of particular Ig and TCR gene segments, however, is tightly regulated with respect to cell lineage and developmental stage. Using an in vitro system, we analyzed recombinase cleavage of RSSs flanking Ig and TCR gene segments in nuclei. We found that both the lineage-specificity and temporal ordering of gene rearrangement is reflected in the accessibility of RSSs within chromatin to in vitro cleavage.
引用
收藏
页码：887 / 897
页数：11
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