Using an innovative pH-stat CO2 feeding strategy to enhance cell growth and C-phycocyanin production from Spirulina platensis

被引：50

作者：

Chen, Chun-Yen ^{[1
]}

Kao, Pei-Chun ^{[2
]}

Tan, Chung Hong ^{[3
]}

Show, Pau Loke ^{[3
,4
]}

Cheah, Wai Yan ^{[5
]}

Lee, Wen-Lung ^{[6
]}

Ling, Tau Chuan ^{[5
]}

Chang, Jo-Shu ^{[2
,7
]}

机构：

[1] Natl Cheng Kung Univ, Univ Ctr Biosci & Biotechnol, Tainan 701, Taiwan

[2] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 701, Taiwan

[3] Univ Nottingham, Fac Engn, Dept Chem & Environm Engn, Malaysia Campus, Semenyih 43500, Selangor Darul, Malaysia

[4] Univ Nottingham, Fac Engn, Ctr Food & Bioprod Proc, Mfg & Ind Proc Div, Malaysia Campus, Semenyih 43500, Selangor Darul, Malaysia

[5] Univ Malaya, Inst Biol Sci, Fac Sci, Kuala Lumpur 50603, Malaysia

[6] Air Force Acad, Dept Math & Phys, Kaohsiung 820, Taiwan

[7] Natl Cheng Kung Univ, Res Ctr Energy Technol & Strategy, Tainan 701, Taiwan

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2016年 / 112卷

关键词：

Microalgae; C-Phycocyanin; pH control system; Chromatography; Optimization; Purification; SCENEDESMUS-OBLIQUUS; WASTE-WATER; EXTRACTION; PURIFICATION; CULTIVATION; ARTHROSPIRA; FIXATION; ANTIOXIDANT; RECOVERY; BIOMASS;

D O I：

10.1016/j.bej.2016.04.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Spirulina platensis can mitigate CO2 emissions and simultaneously produce an antioxidant called C-phycocyanin (C-PC), which is a high value nutraceutical product. An innovative pH control system was used to cultivate S. platensis, in which CO2 feeding, instead of acid/alkaline titration, was used to control the pH of the culture. The optimum culture pH was 9.5. Compared to continuous CO2 feeding system, the pH control system improved the CO2 removal efficiency from 13.6% to 62.3%. The C-PC content and productivity were also enhanced to 16.8% and 0.17 g/L/d, respectively. Therefore, the proposed pH control system is economic and sustainable as it avoids the use of acid/alkaline and reduces the overall CO2 emissions. A two-stage C-PC purification process combining fractional precipitation and ion exchange chromatography could achieve the highest purity (A(615)/A(280)) of 4.33 with a 33% C-PC recovery. (c) 2016 Elsevier B.V. All rights reserved.

引用

页码：78 / 85

页数：8

共 36 条

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